| Literature DB >> 15542192 |
Dongping Jiang1, Gnanasiri S Premachandra, Cliff Johnston, Stanley L Hem.
Abstract
Calcium phosphate adjuvant is a commercially available vaccine adjuvant that potentiates the immune response to antigens. Although its name suggests that it is Ca3(PO4)2, X-ray diffraction, FTIR spectroscopy, thermal analysis and the Ca/P molar ratio identify commercial calcium phosphate adjuvant as non-stoichiometric hydroxyapatite, Ca10-x (HPO4)x (PO4)6-x (OH)2-x, where x varies from 0 to 2. The surface charge is pH-dependent (point of zero charge = 5.5). Consequently, commercial calcium phosphate adjuvant exhibits a negative surface charge at physiological pH and electrostatically adsorbs positively charged antigens. The presence of hydroxyls allows calcium phosphate adjuvant to adsorb phosphorylated antigens by ligand exchange with surface hydroxyls.Entities:
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Year: 2004 PMID： 15542192 DOI： 10.1016/j.vaccine.2004.06.029
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641