Enzyme immobilization onto micro-sized modified PMMA microspheres.

Micro-sized acrolein-modified PMMA (poly[methyl methacrylate]) microspheres were used as the support for enzyme immobilization. The properties of immobilized enzyme as well as the immobilizing behavior were studied. The immobilized amount reached 76.8 mg.g(-1) support, which is relatively high compared with some analogous supports. The K(m) value was larger and the V(max) was smaller in the immobilized form than in the free form. Better resistance of the immobilized enzyme against the temperature, time and pH changes showed that the modified PMMA microspheres could be a good support for immobilization of pepsin.