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Literature DB >> 15536564

Studying the effect of a charged surface on the interaction of bleomycin with DNA using an atomic force microscope.

Olivier Piétrement¹, David Pastré, Fabrice Landousy, Marie-Odile David, Stéphane Fusil, Loïc Hamon, Alain Zozime, Eric Le Cam.

Abstract

The cleavage of DNA caused by the antitumoral drug bleomycin has been investigated using atomic force microscopy (AFM). This work deals with the effect that adsorbing DNA onto a positively- or negatively-charged surface has on the double-strand cleavage of DNA by Fe(III)/bleomycin. Quantitative analysis of the number of breaks per DNA molecule, in bulk and at the surface of the mica substrate, has been performed by analyzing AFM images. It turns out that the cleavage of DNA is strongly inhibited by a positively-charged surface. Our experiments can be interpreted using a simple electrostatic model. This paper is a first step in the study of DNA accessibility to ligand such as bleomycin, using AFM in liquids.

Entities: Chemical Gene

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Year: 2004 PMID： 15536564 DOI： 10.1007/s00249-004-0443-y

Source DB: PubMed Journal: Eur Biophys J ISSN： 0175-7571 Impact factor: 1.733

35 in total

1. Time-lapse imaging of conformational changes in supercoiled DNA by scanning force microscopy.

Authors: Fuji Nagami; Giampaolo Zuccheri; Bruno Samorì; Reiko Kuroda
Journal: Anal Biochem Date: 2002-01-15 Impact factor: 3.365

2. Cleavage of Nucleic Acids by Bleomycin.

Authors: Richard M. Burger
Journal: Chem Rev Date: 1998-05-07 Impact factor: 60.622

3. Structure and chemistry of alkali-labile product formed during Fe(II)-bleomycin-mediated DNA strand scission.

Authors: H Sugiyama; T Morii; I Saito; T Matsuura; S M Hecht
Journal: Nucleic Acids Symp Ser Date: 1986

Review 4. Atomic force microscopy of nucleoprotein complexes.

Authors: Y L Lyubchenko; B L Jacobs; S M Lindsay; A Stasiak
Journal: Scanning Microsc Date: 1995-09

5. Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules.

Authors: D P Allison; P S Kerper; M J Doktycz; J A Spain; P Modrich; F W Larimer; T Thundat; R J Warmack
Journal: Proc Natl Acad Sci U S A Date: 1996-08-20 Impact factor: 11.205

6. Following the assembly of RNA polymerase-DNA complexes in aqueous solutions with the scanning force microscope.

Authors: M Guthold; M Bezanilla; D A Erie; B Jenkins; H G Hansma; C Bustamante
Journal: Proc Natl Acad Sci U S A Date: 1994-12-20 Impact factor: 11.205

Review 7. The role of redox-active metals in the mechanism of action of bleomycin.

Authors: D H Petering; R W Byrnes; W E Antholine
Journal: Chem Biol Interact Date: 1990 Impact factor: 5.192

8. Ascorbate both activates and inactivates bleomycin by free radical generation.

Authors: G R Buettner; P L Moseley
Journal: Biochemistry Date: 1992-10-13 Impact factor: 3.162

9. Adsorption of DNA to mica mediated by divalent counterions: a theoretical and experimental study.

Authors: David Pastré; Olivier Piétrement; Stéphane Fusil; Fabrice Landousy; Josette Jeusset; Marie-Odile David; Loïc Hamon; Eric Le Cam; Alain Zozime
Journal: Biophys J Date: 2003-10 Impact factor: 4.033

10. DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.

Authors: S Ajmera; J C Wu; L Worth; L E Rabow; J Stubbe; J W Kozarich
Journal: Biochemistry Date: 1986-10-21 Impact factor: 3.162

6 in total

Studying the effect of a charged surface on the interaction of bleomycin with DNA using an atomic force microscope.

1. Time-lapse imaging of conformational changes in supercoiled DNA by scanning force microscopy.

2. Cleavage of Nucleic Acids by Bleomycin.

3. Structure and chemistry of alkali-labile product formed during Fe(II)-bleomycin-mediated DNA strand scission.

Review 4. Atomic force microscopy of nucleoprotein complexes.

5. Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules.

6. Following the assembly of RNA polymerase-DNA complexes in aqueous solutions with the scanning force microscope.

Review 7. The role of redox-active metals in the mechanism of action of bleomycin.

8. Ascorbate both activates and inactivates bleomycin by free radical generation.

9. Adsorption of DNA to mica mediated by divalent counterions: a theoretical and experimental study.

10. DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.

1. A new approach to DNA bending by polyamines and its implication in DNA condensation.

2. Enzymatic digestion of single DNA molecules anchored on nanogold-modified surfaces.

3. Investigation on the Binding Mode of 3, 4-Dihydropyrano[c]Chromene Derivative with Double Strand DNA.

4. Raman Research on Bleomycin-Induced DNA Strand Breaks and Repair Processes in Living Cells.

5. Plasmonic hot spots reveal local conformational transitions induced by DNA double-strand breaks.

6. High-resolution AFM imaging of single-stranded DNA-binding (SSB) protein--DNA complexes.