| Literature DB >> 15534229 |
Etsuro Ono1, Keiko Amagai, Satoshi Taharaguchi, Yukiko Tomioka, Saori Yoshino, Yuki Watanabe, Pierre Cherel, Louis-Marie Houdebine, Micheline Adam, Marc Eloit, Manabu Inobe, Toshimitsu Uede.
Abstract
An approach to genetically engineered resistance to pseudorabies virus (PRV) infection was examined by using a transgene encoding a soluble form of nectin-1, also known as herpesvirus entry mediator C. Nectin-1 is an alpha-herpesvirus receptor that binds to virion glycoprotein D. Nectin-1 mediates entry of PRV, herpes simplex virus types 1 and 2, and bovine herpesvirus type 1. To assess the antiviral potential of an ectopic expression of the nectin-1 ectodomain in vivo, six transgenic mouse lines expressing a soluble form of nectin-1, consisting of an extracellular domain of porcine nectin-1 and the Fc portion of human IgG1, were generated. All of the transgenic mouse lines showed nearly complete resistance to PRV infection by means of both i.p. and intranasal routes. These results suggest that the introduction into farm animals of a transgene encoding a soluble form of nectin-1 would offer a potent biological approach to generating alpha-herpesvirus-resistant livestock.Entities:
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Year: 2004 PMID： 15534229 PMCID： PMC528950 DOI： 10.1073/pnas.0405816101
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205