BACKGROUND: To date the direct fresh examination has not been considered, except by a few authors, as a valid laboratory procedure for the diagnosis of cutaneous sporotrichosis. METHODS: Forty-two patients with cutaneous sporotrichosis (36 lymphangitic and six fixed) were studied. Pus was obtained through digital compression of opened lesions followed by collection with a spatula and examination of the material between the slide and the coverslip. Cultures on Sabouraud's glucose agar slants with cloramphenicol and cicloheximide were made in all cases. RESULTS: Direct, fresh examination asteroid bodies of Sporothrix schenckii were found in 85.7% of the cases, which allowed the authors to immediately diagnose the disease. Cultures confirmed the diagnosis in 95.2% of the patients. CONCLUSIONS: Fastness, simplicity and low cost of the fresh examination lead us to recommend systematic use of the method for primary diagnosis of the disease, mainly at first level public health laboratories.
BACKGROUND: To date the direct fresh examination has not been considered, except by a few authors, as a valid laboratory procedure for the diagnosis of cutaneous sporotrichosis. METHODS: Forty-two patients with cutaneous sporotrichosis (36 lymphangitic and six fixed) were studied. Pus was obtained through digital compression of opened lesions followed by collection with a spatula and examination of the material between the slide and the coverslip. Cultures on Sabouraud's glucoseagar slants with cloramphenicol and cicloheximide were made in all cases. RESULTS: Direct, fresh examination asteroid bodies of Sporothrix schenckii were found in 85.7% of the cases, which allowed the authors to immediately diagnose the disease. Cultures confirmed the diagnosis in 95.2% of the patients. CONCLUSIONS: Fastness, simplicity and low cost of the fresh examination lead us to recommend systematic use of the method for primary diagnosis of the disease, mainly at first level public health laboratories.
Authors: Roberto Arenas; Carlos D Sánchez-Cardenas; Lourdes Ramirez-Hobak; Leon Felipe Ruíz Arriaga; Ma Elisa Vega Memije Journal: J Fungi (Basel) Date: 2018-05-23
Authors: Y Zhang; F Hagen; B Stielow; A M Rodrigues; K Samerpitak; X Zhou; P Feng; L Yang; M Chen; S Deng; S Li; W Liao; R Li; F Li; J F Meis; J Guarro; M Teixeira; H S Al-Zahrani; Z Pires de Camargo; L Zhang; G S de Hoog Journal: Persoonia Date: 2015-01-29 Impact factor: 11.051