Literature DB >> 15528568

Selection of single-chain antibodies against the VP8* subunit of rotavirus VP4 outer capsid protein and their expression in Lactobacillus casei.

Vicente Monedero1, Jesús Rodríguez-Díaz, Rosa Viana, Javier Buesa, Gaspar Pérez-Martínez.   

Abstract

Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

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Year:  2004        PMID： 15528568      PMCID： PMC525132          DOI： 10.1128/AEM.70.11.6936-6939.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  24 in total

1.  The repertoire of human germline VH sequences reveals about fifty groups of VH segments with different hypervariable loops.

Authors:  I M Tomlinson; G Walter; J D Marks; M B Llewelyn; G Winter
Journal:  J Mol Biol       Date:  1992-10-05       Impact factor: 5.469

2.  Screening of phage antibody libraries.

Authors:  J L Harrison; S C Williams; G Winter; A Nissim
Journal:  Methods Enzymol       Date:  1996       Impact factor: 1.600

3.  Entry of rotaviruses is a multistep process.

Authors:  E Méndez; S López; M A Cuadras; P Romero; C F Arias
Journal:  Virology       Date:  1999-10-25       Impact factor: 3.616

Review 4.  Single-chain Fvs.

Authors:  R Raag; M Whitlow
Journal:  FASEB J       Date:  1995-01       Impact factor: 5.191

5.  Mapping the hemagglutination domain of rotaviruses.

Authors:  E M Fuentes-Pananá; S López; M Gorziglia; C F Arias
Journal:  J Virol       Date:  1995-04       Impact factor: 5.103

6.  Secretion of biologically active murine interleukin-10 by Lactococcus lactis.

Authors: 
Journal:  Enzyme Microb Technol       Date:  2000-12       Impact factor: 3.493

7.  Therapy of mucosal candidiasis by expression of an anti-idiotype in human commensal bacteria.

Authors:  C Beninati; M R Oggioni; M Boccanera; M R Spinosa; T Maggi; S Conti; W Magliani; F De Bernardis; G Teti; A Cassone; G Pozzi; L Polonelli
Journal:  Nat Biotechnol       Date:  2000-10       Impact factor: 54.908

8.  Antirotavirus immunoglobulin A neutralizes virus in vitro after transcytosis through epithelial cells and protects infant mice from diarrhea.

Authors:  F M Ruggeri; K Johansen; G Basile; J P Kraehenbuhl; L Svensson
Journal:  J Virol       Date:  1998-04       Impact factor: 5.103

9.  Cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from Lactobacillus paracasei subsp. paracasei NCDO 151.

Authors:  A Holck; H Naes
Journal:  J Gen Microbiol       Date:  1992-07

Review 10.  Lactic acid bacteria as live vaccines.

Authors:  A Mercenier; H Müller-Alouf; C Grangette
Journal:  Curr Issues Mol Biol       Date:  2000-01       Impact factor: 2.081
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  4 in total

1.  A combination of scGOS/lcFOS with Bifidobacterium breve M-16V protects suckling rats from rotavirus gastroenteritis.

Authors:  M Rigo-Adrover; S Saldaña-Ruíz; K van Limpt; K Knipping; J Garssen; J Knol; A Franch; M Castell; F J Pérez-Cano
Journal:  Eur J Nutr       Date:  2016-04-25       Impact factor: 5.614

Review 2.  Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy.

Authors:  Kristian Daniel Ralph Roth; Esther Veronika Wenzel; Maximilian Ruschig; Stephan Steinke; Nora Langreder; Philip Alexander Heine; Kai-Thomas Schneider; Rico Ballmann; Viola Fühner; Philipp Kuhn; Thomas Schirrmann; André Frenzel; Stefan Dübel; Maren Schubert; Gustavo Marçal Schmidt Garcia Moreira; Federico Bertoglio; Giulio Russo; Michael Hust
Journal:  Front Cell Infect Microbiol       Date:  2021-07-07       Impact factor: 5.293

3.  Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants.

Authors:  Paloma Juarez; Estefania Huet-Trujillo; Alejandro Sarrion-Perdigones; Erica Elvira Falconi; Antonio Granell; Diego Orzaez
Journal:  Int J Mol Sci       Date:  2013-03-18       Impact factor: 5.923

Review 4.  Recombinant antibodies for diagnostics and therapy against pathogens and toxins generated by phage display.

Authors:  Philipp Kuhn; Viola Fühner; Tobias Unkauf; Gustavo Marcal Schmidt Garcia Moreira; André Frenzel; Sebastian Miethe; Michael Hust
Journal:  Proteomics Clin Appl       Date:  2016-06-21       Impact factor: 3.494
  4 in total

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