| Literature DB >> 15527787 |
Tatiana V Ovchinnikova1, Galina M Aleshina, Sergey V Balandin, Anna D Krasnosdembskaya, Mikhail L Markelov, Elena I Frolova, Yulia F Leonova, Andrey A Tagaev, Eugeny G Krasnodembsky, Vladimir N Kokryakov.
Abstract
Two novel 21-residue antimicrobial peptides, arenicin-1 and arenicin-2, exhibiting activity against Gram-positive and Gram-negative bacteria and fungi, were purified from coelomocytes of marine polychaeta Arenicola marina (lugworm) by preparative gel electrophoresis and RP-HPLC. Molecular masses (2758.3 and 2772.3 Da) and complete amino acid sequences (RWCVYAYVRVRGVLVRYRRCW and RWCVYAYVRIRGVLVRYRRCW) were determined for each isoform. Each arenicin has one disulfide bond (Cys3-Cys20). The total RNA was isolated from the lugworm coelomocytes, RT-PCR and cloning were performed, and cDNA was sequenced. A 202-residue preproarenicin contains a putative signal peptide (25 amino acids) and a long prodomain. Arenicins have no structure similarity to any previously identified antimicrobial peptides.Entities:
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Year: 2004 PMID： 15527787 DOI： 10.1016/j.febslet.2004.10.012
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124