Literature DB >> 15519948

Recombinant adeno-associated virus serotype 2 effectively transduces primary rat brain astrocytes and microglia.

Yan Gong1, Shuzhen Chen, Christopher F Sonntag, Colin Sumners, Ronald L Klein, Michael A King, Jeffrey A Hughes, Edwin M Meyer.   

Abstract

Recombinant adeno-associated virus-2 (rAAV2) under control of the chicken beta actin promoter/truncated CMV enhancer (CBA) was investigated for its ability to transduce primary cultures of rat brain neurons, microglia and astrocytes. This vector was highly effective in all three cell types in heparin-sensitive manners (astrocytes, microglia and neurons transduced by >98%, 75%, and 95%, respectively). However, astrocytes co-cultured with neurons were not transduced. rAAV2/CBA is an important new method for genetic manipulation of brain cells, though this may be modulated by interactions among cell types.

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Year:  2004        PMID： 15519948     DOI： 10.1016/j.brainresprot.2004.08.001

Source DB:  PubMed          Journal:  Brain Res Brain Res Protoc        ISSN： 1385-299X


  8 in total

1.  Efficient neuronal gene transfer with AAV8 leads to neurotoxic levels of tau or green fluorescent proteins.

Authors:  Ronald L Klein; Robert D Dayton; Nancy J Leidenheimer; Karen Jansen; Todd E Golde; Richard M Zweig
Journal:  Mol Ther       Date:  2005-12-01       Impact factor: 11.454

2.  Tropism and toxicity of adeno-associated viral vector serotypes 1, 2, 5, 6, 7, 8, and 9 in rat neurons and glia in vitro.

Authors:  Douglas B Howard; Kathleen Powers; Yun Wang; Brandon K Harvey
Journal:  Virology       Date:  2007-11-26       Impact factor: 3.616

3.  Improved Adeno-associated Viral Gene Transfer to Murine Glioma.

Authors:  I Zolotukhin; D Luo; Os Gorbatyuk; Be Hoffman; Kh Warrington; Rw Herzog; Jk Harrison; O Cao
Journal:  J Genet Syndr Gene Ther       Date:  2013-04-29

4.  AAV8, 9, Rh10, Rh43 vector gene transfer in the rat brain: effects of serotype, promoter and purification method.

Authors:  Ronald L Klein; Robert D Dayton; Jason B Tatom; Karen M Henderson; Phillip P Henning
Journal:  Mol Ther       Date:  2007-10-23       Impact factor: 11.454

5.  Macrophage migration inhibitory factor in hypothalamic paraventricular nucleus neurons decreases blood pressure in spontaneously hypertensive rats.

Authors:  Hongwei Li; Yongxin Gao; Yanfei Qi; Michael J Katovich; Nan Jiang; Leah N Braseth; Deborah A Scheuer; Peng Shi; Colin Sumners
Journal:  FASEB J       Date:  2008-06-05       Impact factor: 5.191

6.  Specific AAV serotypes stably transduce primary hippocampal and cortical cultures with high efficiency and low toxicity.

Authors:  Nicolas C Royo; Luk H Vandenberghe; Jing-Yuan Ma; Alisse Hauspurg; Liya Yu; Margaret Maronski; Julie Johnston; Marc A Dichter; James M Wilson; Deborah J Watson
Journal:  Brain Res       Date:  2007-11-17       Impact factor: 3.252

7.  Development of Efficient AAV2/DJ-Based Viral Vectors to Selectively Downregulate the Expression of Neuronal or Astrocytic Target Proteins in the Rat Central Nervous System.

Authors:  Charlotte Jollé; Nicole Déglon; Catherine Pythoud; Anne-Karine Bouzier-Sore; Luc Pellerin
Journal:  Front Mol Neurosci       Date:  2019-08-20       Impact factor: 5.639

8.  Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors.

Authors:  Nicolas Merienne; Juliette Le Douce; Emilie Faivre; Nicole Déglon; Gilles Bonvento
Journal:  Front Cell Neurosci       Date:  2013-07-05       Impact factor: 5.505
  8 in total

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