BACKGROUND: Perillyl alcohol (POH) elicited anticarcinogenic effects in a number of cancer models and pharmacokinetic studies in humans revealed that PA is the major circulating metabolite following POH administration. MATERIALS AND METHODS: Effects of PA or POH alone, or in combination with radiation, on human head and neck squamous cell carcinoma cell lines (HNSCC), were investigated using cytotoxicity and flow cytometry assays. HNSCC cells were pretreated with 1.0 mM PA or 0.5 mM POH for 72 h before exposure to 1 or 2Gy dose of radiation. RESULTS: Pretreatment of the cells with 1.0 mM PA or 0.5 mM POH prior to irradiation, caused the following growth inhibition: HTB-43 (50% or 71%), SCC-25 (55% or 68%), and BroTo (18% or 53%). PA and POH induced cell-cycle arrest and apoptosis. CONCLUSION: PA and POH have potential for use as radiosensitizers in chemo-radiation therapy of head and neck cancers and should be further studied.
BACKGROUND:Perillyl alcohol (POH) elicited anticarcinogenic effects in a number of cancer models and pharmacokinetic studies in humans revealed that PA is the major circulating metabolite following POH administration. MATERIALS AND METHODS: Effects of PA or POH alone, or in combination with radiation, on human head and neck squamous cell carcinoma cell lines (HNSCC), were investigated using cytotoxicity and flow cytometry assays. HNSCC cells were pretreated with 1.0 mM PA or 0.5 mM POH for 72 h before exposure to 1 or 2Gy dose of radiation. RESULTS: Pretreatment of the cells with 1.0 mM PA or 0.5 mM POH prior to irradiation, caused the following growth inhibition: HTB-43 (50% or 71%), SCC-25 (55% or 68%), and BroTo (18% or 53%). PA and POH induced cell-cycle arrest and apoptosis. CONCLUSION: PA and POH have potential for use as radiosensitizers in chemo-radiation therapy of head and neck cancers and should be further studied.
Authors: Paul Yaswen; Karen L MacKenzie; W Nicol Keith; Patricia Hentosh; Francis Rodier; Jiyue Zhu; Gary L Firestone; Ander Matheu; Amancio Carnero; Alan Bilsland; Tabetha Sundin; Kanya Honoki; Hiromasa Fujii; Alexandros G Georgakilas; Amedeo Amedei; Amr Amin; Bill Helferich; Chandra S Boosani; Gunjan Guha; Maria Rosa Ciriolo; Sophie Chen; Sulma I Mohammed; Asfar S Azmi; Dipita Bhakta; Dorota Halicka; Elena Niccolai; Katia Aquilano; S Salman Ashraf; Somaira Nowsheen; Xujuan Yang Journal: Semin Cancer Biol Date: 2015-04-11 Impact factor: 15.707
Authors: Sareh Kamran; Ajantha Sinniah; Mahfoudh A M Abdulghani; Mohammed Abdullah Alshawsh Journal: Cancers (Basel) Date: 2022-02-22 Impact factor: 6.639