BACKGROUND: Multidrug resistance associated proteins MRP1, MRP2 and MRP3 confer in vitro multidrug resistance. We investigated their role in breast cancer resistance to anthracycline-based chemotherapy. MATERIALS AND METHODS: Using real-time reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC), the expression of MRP1 - 3 was quantified in nine breast cancer cell lines and 30 breast carcinoma samples. RESULTS: MRP1 - 3 mRNA was detectable in all breast cancer cell lines and tumor samples. No increase of expression was detected between untreated carcinoma and post-neoadjuvant anthracycline treatment tumor samples. IHC failed to detect the proteins. MRP1 - 3 expression was not associated with tumor response to treatment or with outcome. CONCLUSION: MRP1 - 3 are expressed in breast cancer cells, but are not detected with IHC. We have found no evidence linking these proteins to clinical drug resistance in a small but well-documented series of breast cancer samples.
BACKGROUND: Multidrug resistance associated proteins MRP1, MRP2 and MRP3 confer in vitro multidrug resistance. We investigated their role in breast cancer resistance to anthracycline-based chemotherapy. MATERIALS AND METHODS: Using real-time reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC), the expression of MRP1 - 3 was quantified in nine breast cancer cell lines and 30 breast carcinoma samples. RESULTS:MRP1 - 3 mRNA was detectable in all breast cancer cell lines and tumor samples. No increase of expression was detected between untreated carcinoma and post-neoadjuvant anthracycline treatment tumor samples. IHC failed to detect the proteins. MRP1 - 3 expression was not associated with tumor response to treatment or with outcome. CONCLUSION:MRP1 - 3 are expressed in breast cancer cells, but are not detected with IHC. We have found no evidence linking these proteins to clinical drug resistance in a small but well-documented series of breast cancer samples.
Authors: Marie Ehrlichova; Radka Vaclavikova; Iwao Ojima; Antonella Pepe; Larisa V Kuznetsova; Jin Chen; Jaroslav Truksa; Jan Kovar; Ivan Gut Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 2005-07-30 Impact factor: 3.000
Authors: Silvia Rybárová; Janka Vecanová; Ingrid Hodorová; Jozef Mihalik; Martina Čižmáriková; Ján Mojžiš; Peter Solár; Marián Benický; Marian Adamkov; Ladislav Mirossay Journal: Med Sci Monit Date: 2011-12
Authors: Mario Martínez Soldevilla; Helena Villanueva; Noelia Casares; Juan Jose Lasarte; Maurizio Bendandi; Susana Inoges; Ascensión López-Díaz de Cerio; Fernando Pastor Journal: Oncotarget Date: 2016-04-26