Literature DB >> 15516633

Short report: fluorescent Leishmania: application to anti-leishmanial drug testing.

Neeloo Singh1, Anuradha Dube.   

Abstract

Classic techniques for detecting the susceptibility of Leishmania to different drugs are time-consuming, laborious, and require the use of macrophages. The use of flow cytometry for monitoring Leishmania susceptibility to drugs is beginning to be implemented. Using green fluorescent protein (GFP), we have improved and simplified the screening procedure. We introduced a GFP marker into field strains of Leishmania causing kala-azar (visceral leishmaniasis) and explored the suitability of transgenic L. donovani promastigotes that constitutively express GFP in their cytoplasm as target cells for in vitro screening of anti-leishmanial drugs.

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Year:  2004        PMID： 15516633

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN： 0002-9637            Impact factor:   2.345


  21 in total

1.  Translation of open reading frame in kinetoplast DNA minicircles of clinical isolates of L. donovani.

Authors:  Hema Kothari; Pranav Kumar; Rohit Saluja; Shyam Sundar; Neeloo Singh
Journal:  Parasitol Res       Date:  2006-10-18       Impact factor: 2.289

2.  Immunostimulatory cellular responses of cured Leishmania-infected patients and hamsters against the integral membrane proteins and non-membranous soluble proteins of a recent clinical isolate of Leishmania donovani.

Authors:  R Garg; S K Gupta; P Tripathi; S Naik; S Sundar; A Dube
Journal:  Clin Exp Immunol       Date:  2005-04       Impact factor: 4.330

3.  Refractoriness to the treatment of sodium stibogluconate in Indian kala-azar field isolates persist in in vitro and in vivo experimental models.

Authors:  Anuradha Dube; Nasib Singh; Shyam Sundar; Neeloo Singh
Journal:  Parasitol Res       Date:  2005-05-03       Impact factor: 2.289

4.  Monitoring the efficacy of antimicrobial photodynamic therapy in a murine model of cutaneous leishmaniasis using L. major expressing GFP.

Authors:  Elena Latorre-Esteves; Oleg E Akilov; Prakash Rai; Stephen M Beverley; Tayyaba Hasan
Journal:  J Biophotonics       Date:  2010-06       Impact factor: 3.207

5.  In silico screening, structure-activity relationship, and biologic evaluation of selective pteridine reductase inhibitors targeting visceral leishmaniasis.

Authors:  Jaspreet Kaur; Pranav Kumar; Sargam Tyagi; Richa Pathak; Sanjay Batra; Prashant Singh; Neeloo Singh
Journal:  Antimicrob Agents Chemother       Date:  2010-11-29       Impact factor: 5.191

6.  Antileishmanial activity in vitro and in vivo of constituents of sea cucumber Actinopyga lecanora.

Authors:  Nasib Singh; Rajesh Kumar; Swati Gupta; Anuradha Dube; V Lakshmi
Journal:  Parasitol Res       Date:  2008-05-02       Impact factor: 2.289

7.  An orally effective dihydropyrimidone (DHPM) analogue induces apoptosis-like cell death in clinical isolates of Leishmania donovani overexpressing pteridine reductase 1.

Authors:  Neeloo Singh; Jaspreet Kaur; Pranav Kumar; Swati Gupta; Nasib Singh; Angana Ghosal; Avijit Dutta; Ashutosh Kumar; Ramapati Tripathi; Mohammad Imran Siddiqi; Chitra Mandal; Anuradha Dube
Journal:  Parasitol Res       Date:  2009-07-21       Impact factor: 2.289

8.  An alternative in vitro drug screening test using Leishmania amazonensis transfected with red fluorescent protein.

Authors:  Marcele N Rocha; Célia M Corrêa; Maria N Melo; Stephen M Beverley; Olindo Assis Martins-Filho; Ana Paula Madureira; Rodrigo P Soares
Journal:  Diagn Microbiol Infect Dis       Date:  2013-01-10       Impact factor: 2.803

9.  Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery.

Authors:  Sanjay R Mehta; Robert Huang; Meng Yang; Xing-Quan Zhang; Bala Kolli; Kwang-Poo Chang; Robert M Hoffman; Yasuyuki Goto; Roberto Badaro; Robert T Schooley
Journal:  Clin Vaccine Immunol       Date:  2008-10-22

Review 10.  Visceral leishmaniasis treatment: What do we have, what do we need and how to deliver it?

Authors:  Lucio H Freitas-Junior; Eric Chatelain; Helena Andrade Kim; Jair L Siqueira-Neto
Journal:  Int J Parasitol Drugs Drug Resist       Date:  2012-01-28       Impact factor: 4.077
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