Literature DB >> 1551598

Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine:D-alanine ligase-related protein necessary for vancomycin resistance.

S Dutka-Malen1, C Molinas, M Arthur, P Courvalin.   

Abstract

The amplification product obtained with DNA from vancomycin-resistant (VmR) Enterococcus gallinarum BM4174 and a pair of degenerate oligodeoxyribonucleotides that correspond to conserved amino acid (aa) motifs in Escherichia coli D-alanine (D-Ala):D-Ala ligases and in En. faecium VmR protein (VanA) was used as a probe to clone the vanC gene of that strain. The vanC product, with a calculated Mr of 37,504, exhibits 29 to 38% aa identity with VanA and E. coli ligases. Insertional inactivation of vanC led to Vm sensitivity of BM4174 suggesting that the gene may encode a D-Ala:D-Ala ligase of altered specificity.

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Year:  1992        PMID： 1551598     DOI： 10.1016/0378-1119(92)90302-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  55 in total

1.  The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).

Authors:  D I Roper; T Huyton; A Vagin; G Dodson
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-01       Impact factor: 11.205

2.  Molecular characterization of the vanE gene cluster in vancomycin-resistant Enterococcus faecalis N00-410 isolated in Canada.

Authors:  D A Boyd; T Cabral; P Van Caeseele; J Wylie; M R Mulvey
Journal:  Antimicrob Agents Chemother       Date:  2002-06       Impact factor: 5.191

3.  Vancomycin resistance is associated with serine-containing peptidoglycan in Enterococcus gallinarum.

Authors:  P Grohs; L Gutmann; R Legrand; B Schoot; J L Mainardi
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

Review 4.  Vancomycin resistance in enterococci due to synthesis of precursors terminating in D-alanyl-D-serine.

Authors:  Peter E Reynolds; Patrice Courvalin
Journal:  Antimicrob Agents Chemother       Date:  2005-01       Impact factor: 5.191

5.  VanG-type vancomycin-resistant Enterococcus faecalis strains isolated in Canada.

Authors:  David A Boyd; Tim Du; Romeo Hizon; Brynn Kaplen; Travis Murphy; Shaun Tyler; Shirley Brown; Frances Jamieson; Karl Weiss; Michael R Mulvey
Journal:  Antimicrob Agents Chemother       Date:  2006-06       Impact factor: 5.191

6.  Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci.

Authors:  R Patel; J R Uhl; P Kohner; M K Hopkins; F R Cockerill
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

7.  D-Ala:D-Ala ligase gene flanking the vanC cluster: evidence for presence of three ligase genes in vancomycin-resistant Enterococcus gallinarum BM4174.

Authors:  Ole-Herman Ambúr; Peter E Reynolds; Cesar A Arias
Journal:  Antimicrob Agents Chemother       Date:  2002-01       Impact factor: 5.191

8.  Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR.

Authors:  S Dutka-Malen; S Evers; P Courvalin
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

9.  Biochemical and genetic characterization of the vanC-2 vancomycin resistance gene cluster of Enterococcus casseliflavus ATCC 25788.

Authors:  Ireena Dutta; Peter E Reynolds
Journal:  Antimicrob Agents Chemother       Date:  2002-10       Impact factor: 5.191

10.  Analysis of peptidoglycan precursors in vancomycin-resistant Enterococcus gallinarum BM4174.

Authors:  P E Reynolds; H A Snaith; A J Maguire; S Dutka-Malen; P Courvalin
Journal:  Biochem J       Date:  1994-07-01       Impact factor: 3.857
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