Literature DB >> 15514791

Intein-mediated purification of a recombinantly expressed peptide.

John A Pezza1, Karen N Allen, Dean R Tolan.   

Abstract

A 26 amino acid peptide has successfully been purified via recombinant expression as an intein fusion protein accompanied by cleavage without the need for any exogenous proteases or cofactors, thus offering a practical, inexpensive approach to produce isotopically labelled peptides.

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Year:  2004        PMID: 15514791     DOI: 10.1039/b407924h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  6 in total

1.  Semisynthesis of a glycosylated Im7 analogue for protein folding studies.

Authors:  Christian P R Hackenberger; Claire T Friel; Sheena E Radford; Barbara Imperiali
Journal:  J Am Chem Soc       Date:  2005-09-21       Impact factor: 15.419

2.  Thermodynamic analysis shows conformational coupling and dynamics confer substrate specificity in fructose-1,6-bisphosphate aldolase.

Authors:  John A Pezza; Jack D Stopa; Elizabeth M Brunyak; Karen N Allen; Dean R Tolan
Journal:  Biochemistry       Date:  2007-10-13       Impact factor: 3.162

3.  Expression and one step intein-mediated purification of biologically active human G-CSF in Escherichia coli.

Authors:  Setareh Sima; Fatemeh Shafiee; Ali Jahanian-Najafabadi
Journal:  Mol Biol Rep       Date:  2020-03-29       Impact factor: 2.316

4.  Purification of Antibacterial CHAPK Protein Using a Self-Cleaving Fusion Tag and Its Activity Against Methicillin-Resistant Staphylococcus aureus.

Authors:  Elahe Seyed Hosseini; Rezvan Moniri; Yasaman Dasteh Goli; Hamed Haddad Kashani
Journal:  Probiotics Antimicrob Proteins       Date:  2016-12       Impact factor: 4.609

Review 5.  Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli.

Authors:  Yifeng Li
Journal:  Biotechnol Appl Biochem       Date:  2009-07-06       Impact factor: 2.431

6.  Optimization and One-Step Purification of Recombinant V Antigen Production from Yersinia pestis.

Authors:  Elahe Seyed Hosseini; Mehdi Zeinoddini; Ali Reza Saeedinia; Valiollah Babaeipour
Journal:  Mol Biotechnol       Date:  2020-03       Impact factor: 2.695

  6 in total

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