AIM: To evaluate the prevalence of antibodies against two major markers of virulence of Helicobacter pylori--cytotoxin-associated gene A (cagA) and the vacuolating cytotoxin gene (vacA)--among children in a peri-urban community of Bangladesh, and to evaluate Western blot (WB) assay for detection of H. pylori infection diagnosed by 13C urea breath test (UBT) in such children. METHODS: One hundred and eighty-two children aged 18-60 mo, of the peri-urban community of Dhaka, were screened for H. pylori infection using UBT, and the serum samples were analysed for antibody against cagA and vacA by Western blot. RESULTS: The overall prevalence of H. pylori infection by 13C-urea breath test was 80%. The seroprevalence of cagA with or without vacA, vacA with and without cagA, and both cagA and vacA were 82%, 82% and 81%, respectively. Among children with a positive UBT, 95% were seropositive for both cagA and vacA, indicating that the products of these genes are frequently co-expressed in H. pylori infection in this community. The sensitivity, specificity, positive and negative predictive value of the Western blot test for H. pylori infections, compared to UBT, were 94%, 68%, 92% and 76%, respectively. CONCLUSION: Compared to UBT, Western blot test is reliable for the detection of H. pylori infection. The high seroprevalence of cagA- and vacA-positive virulent H. pylori strains in an asymptomatic paediatric population indicate that such strains are common in this population and may cause characteristic H. pylori infection in Bangladesh.
AIM: To evaluate the prevalence of antibodies against two major markers of virulence of Helicobacter pylori--cytotoxin-associated gene A (cagA) and the vacuolating cytotoxin gene (vacA)--among children in a peri-urban community of Bangladesh, and to evaluate Western blot (WB) assay for detection of H. pyloriinfection diagnosed by 13Curea breath test (UBT) in such children. METHODS: One hundred and eighty-two children aged 18-60 mo, of the peri-urban community of Dhaka, were screened for H. pyloriinfection using UBT, and the serum samples were analysed for antibody against cagA and vacA by Western blot. RESULTS: The overall prevalence of H. pyloriinfection by 13C-urea breath test was 80%. The seroprevalence of cagA with or without vacA, vacA with and without cagA, and both cagA and vacA were 82%, 82% and 81%, respectively. Among children with a positive UBT, 95% were seropositive for both cagA and vacA, indicating that the products of these genes are frequently co-expressed in H. pyloriinfection in this community. The sensitivity, specificity, positive and negative predictive value of the Western blot test for H. pylori infections, compared to UBT, were 94%, 68%, 92% and 76%, respectively. CONCLUSION: Compared to UBT, Western blot test is reliable for the detection of H. pyloriinfection. The high seroprevalence of cagA- and vacA-positive virulent H. pylori strains in an asymptomatic paediatric population indicate that such strains are common in this population and may cause characteristic H. pyloriinfection in Bangladesh.
Authors: S Nahar; K M K Kibria; Md E Hossain; J Sultana; S A Sarker; L Engstrand; P K Bardhan; M Rahman; H P Endtz Journal: Eur J Clin Microbiol Infect Dis Date: 2009-02-04 Impact factor: 3.267
Authors: Cezary Chojnacki; Tomasz Popławski; Aleksandra Błońska; Janusz Błasiak; Marek Romanowski; Jan Chojnacki Journal: Arch Med Sci Date: 2018-07-05 Impact factor: 3.318
Authors: Yelda A Leal; Laura L Flores; Laura B García-Cortés; Roberto Cedillo-Rivera; Javier Torres Journal: PLoS One Date: 2008-11-18 Impact factor: 3.240