Literature DB >> 15509020

A preliminary trial using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) on the same feedstuffs to detect tissues of animal origin.

F Colombo1, E Marchisio, I E Trezzi, V Peri, L Pinotti, A Baldi, G Soncini.   

Abstract

A preliminary study using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) was done on the same feedstuffs to detect animal tissues. The results of the two methods differ somewhat: PCR-RFLP did not detect any signal in any sample, but multiplex PCR detected a signal in one sample. These findings could be a basis for further investigations.

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Year:  2004        PMID: 15509020     DOI: 10.1023/b:verc.0000040248.52246.82

Source DB:  PubMed          Journal:  Vet Res Commun        ISSN: 0165-7380            Impact factor:   2.459


  8 in total

1.  Identification of species in animal feedstuffs by polymerase chain reaction--restriction fragment length polymorphism analysis of mitochondrial DNA.

Authors:  F Bellagamba; V M Moretti; S Comincini; F Valfrè
Journal:  J Agric Food Chem       Date:  2001-08       Impact factor: 5.279

2.  A PCR-based test for species-specific determination of heat treatment conditions of animal meals as an effective prophylactic method for bovine spongiform encephalopathy.

Authors:  C I Kingombe; E Lüthi; H Schlosser; D Howald; M Kuhn; T Jemmi
Journal:  Meat Sci       Date:  2001-01       Impact factor: 5.209

3.  A quick and simple method for the identification of meat species and meat products by PCR assay.

Authors:  T Matsunaga; K Chikuni; R Tanabe; S Muroya; K Shibata; J Yamada; Y Shinmura
Journal:  Meat Sci       Date:  1999-02       Impact factor: 5.209

4.  Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.

Authors:  T D Kocher; W K Thomas; A Meyer; S V Edwards; S Pääbo; F X Villablanca; A C Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

5.  Variations in mitochondrial tRNA gene organization of reptiles as phylogenetic markers.

Authors:  Y Kumazawa; M Nishida
Journal:  Mol Biol Evol       Date:  1995-09       Impact factor: 16.240

6.  Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section Flavi.

Authors:  Y Kumeda; T Asao
Journal:  Appl Environ Microbiol       Date:  1996-08       Impact factor: 4.792

7.  Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food.

Authors:  R Meyer; C Höfelein; J Lüthy; U Candrian
Journal:  J AOAC Int       Date:  1995 Nov-Dec       Impact factor: 1.913

8.  Detection of bovine mitochondrial DNA in ruminant feeds: a molecular approach to test for the presence of bovine-derived materials.

Authors:  M Tartaglia; E Saulle; S Pestalozza; L Morelli; G Antonucci; P A Battaglia
Journal:  J Food Prot       Date:  1998-05       Impact factor: 2.077

  8 in total

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