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Literature DB >> 15507717

Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.

Toh Hean Ch'ng¹, E Alexander Flood, Lynn William Enquist.

Abstract

This chapter discusses the culture of primary sympathetic neurons (superior cervical ganglia) from rat embryos and PC12 cells differentiated into neurons for use in viral infection experiments. Methods are described for the use of a neurotropic herpesvirus, pseudorabies virus (PRV), to analyze the assembly, egress, and transport of viral antigens in neurons.

Entities: Disease Species

Mesh：

Year: 2005 PMID： 15507717 DOI： 10.1385/1-59259-848-x:299

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

33 in total

1. Herpes simplex virus type 1 glycoprotein e is required for axonal localization of capsid, tegument, and membrane glycoproteins.

Authors: Fushan Wang; Waixing Tang; Helen M McGraw; Jean Bennett; Lynn W Enquist; Harvey M Friedman
Journal: J Virol Date: 2005-11 Impact factor: 5.103

2. Neuron-to-cell spread of pseudorabies virus in a compartmented neuronal culture system.

Authors: T H Ch'ng; L W Enquist
Journal: J Virol Date: 2005-09 Impact factor: 5.103

3. Compartmented neuron cultures for directional infection by alpha herpesviruses.

Authors: Dusica Curanović; Toh Hean Ch'ng; Moriah Szpara; Lynn Enquist
Journal: Curr Protoc Cell Biol Date: 2009-06

4. Glycoproteins gE and gI are required for efficient KIF1A-dependent anterograde axonal transport of alphaherpesvirus particles in neurons.

Authors: Radomir Kratchmarov; Tal Kramer; Todd M Greco; Matthew P Taylor; Toh Hean Ch'ng; Ileana M Cristea; Lynn W Enquist
Journal: J Virol Date: 2013-06-26 Impact factor: 5.103

5. Efficient axonal localization of alphaherpesvirus structural proteins in cultured sympathetic neurons requires viral glycoprotein E.

Authors: T H Ch'ng; L W Enquist
Journal: J Virol Date: 2005-07 Impact factor: 5.103

Culturing primary and transformed neuronal cells for studying pseudorabies virus infection.

1. Herpes simplex virus type 1 glycoprotein e is required for axonal localization of capsid, tegument, and membrane glycoproteins.

2. Neuron-to-cell spread of pseudorabies virus in a compartmented neuronal culture system.

3. Compartmented neuron cultures for directional infection by alpha herpesviruses.

4. Glycoproteins gE and gI are required for efficient KIF1A-dependent anterograde axonal transport of alphaherpesvirus particles in neurons.

5. Efficient axonal localization of alphaherpesvirus structural proteins in cultured sympathetic neurons requires viral glycoprotein E.

6. Kinesin-3 mediates axonal sorting and directional transport of alphaherpesvirus particles in neurons.

7. Pseudorabies virus infection alters neuronal activity and connectivity in vitro.

8. Virion-incorporated glycoprotein B mediates transneuronal spread of pseudorabies virus.

9. Herpes simplex virus type 1 glycoprotein E mediates retrograde spread from epithelial cells to neurites.

10. In vitro analysis of transneuronal spread of an alphaherpesvirus infection in peripheral nervous system neurons.