| Literature DB >> 15507398 |
Tomonori Nochi1, Yoshikazu Yuki, Kazutaka Terahara, Ayako Hino, Jun Kunisawa, Mi-Na Kweon, Takahiro Yamaguchi, Hiroshi Kiyono.
Abstract
The mucosal epithelium including intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) provide a first line of defense in the gastrointestinal tract. However, limited information is currently available concerning the nature of the physical interaction molecule that interconnects IECs and IELs. Among the several monoclonal antibodies (mAbs) generated by immunizing porcine IECs, mAb (5-15-1) was shown to strongly react with IELs in addition to IECs. MALDI-TOF-MS and tandem MS analysis suggested that the antigen belongs to a family of human homophilic epithelial cell adhesion molecule (Ep-CAM). The amino acid sequence of porcine Ep-CAM showed 82.8%, 78.1%, and 76.8% homology compared to human, mouse, and rat Ep-CAM. Moreover, 5-15-1 specifically reacted with transfectant of porcine Ep-CAM. These data suggest that the Ep-CAM may act as a physical homophilic interaction molecule between IELs and IECs at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection.Entities:
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Year: 2004 PMID： 15507398 DOI： 10.1016/j.clim.2004.08.013
Source DB: PubMed Journal: Clin Immunol ISSN： 1521-6616 Impact factor: 3.969