OBJECTIVE: To determine the content of isopimpinellin in root of Toddalia asiatica. METHODS: A HPLC method was set up. Using Hypersil C18 column and methanol-water (70:30) as mobile phase, with the detection wavelength at 306 nm. RESULT: The linear range of isopimpinellin was 0.004 20 approximately 0.420 microg. The average recovery was 99.7% and the RSD 2.8%. CONCLUSION: The method is simple and accurate, with good reproducibility, and can be used as a quantitative analysis method for isopimpinellin.
OBJECTIVE: To determine the content of isopimpinellin in root of Toddalia asiatica. METHODS: A HPLC method was set up. Using Hypersil C18 column and methanol-water (70:30) as mobile phase, with the detection wavelength at 306 nm. RESULT: The linear range of isopimpinellin was 0.004 20 approximately 0.420 microg. The average recovery was 99.7% and the RSD 2.8%. CONCLUSION: The method is simple and accurate, with good reproducibility, and can be used as a quantitative analysis method for isopimpinellin.