Literature DB >> 1550568

The role of the exocyclic amino groups of conserved purines in hammerhead ribozyme cleavage.

G Slim1, M J Gait.   

Abstract

A series of 2 stranded hammerhead ribozymes has been synthesized in which single conserved adenosine residues have been replaced by nebularine or single guanosine residues by inosine. Comparison of the rates of trans-cleavage for the modified structures suggests the presence of interstrand non-Watson-Crick hydrogen bonding interactions including a GA:AG double mismatch. The exocyclic amino group of one of the guanosine residues is essential for cleavage.

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Year:  1992        PMID： 1550568     DOI： 10.1016/0006-291x(92)90525-p

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

1.  Activity of hammerhead ribozymes containing non-nucleotidic linkers.

Authors:  J B Thomson; T Tuschl; F Eckstein
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

2.  Structure-function relationships in the hammerhead ribozyme probed by base rescue.

Authors:  A Peracchi; J Matulic-Adamic; S Wang; L Beigelman; D Herschlag
Journal:  RNA       Date:  1998-11       Impact factor: 4.942

Review 3.  Hammerhead ribozyme kinetics.

Authors:  T K Stage-Zimmermann; O C Uhlenbeck
Journal:  RNA       Date:  1998-08       Impact factor: 4.942

4.  1-Deazaadenosine: synthesis and activity of base-modified hammerhead ribozymes.

Authors:  F Seela; H Debelak; N Usman; A Burgin; L Beigelman
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

5.  Probing the hammerhead ribozyme structure with ribonucleases.

Authors:  R A Hodgson; N J Shirley; R H Symons
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

6.  Hammerhead ribozymes: importance of stem-loop II for activity.

Authors:  T Tuschl; F Eckstein
Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-01       Impact factor: 11.205

7.  The synthesis of oligoribonucleotides containing O6-methylguanosine: the role of conserved guanosine residues in hammerhead ribozyme cleavage.

Authors:  J A Grasby; P Jonathan; G Butler; M J Gait
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

8.  The loss of a hydrogen bond: Thermodynamic contributions of a non-standard nucleotide.

Authors:  Elizabeth A Jolley; Brent M Znosko
Journal:  Nucleic Acids Res       Date:  2017-02-17       Impact factor: 16.971

9.  The roles of the conserved pyrimidine bases in hammerhead ribozyme catalysis: evidence for a magnesium ion-binding site.

Authors:  J B Murray; C J Adams; J R Arnold; P G Stockley
Journal:  Biochem J       Date:  1995-10-15       Impact factor: 3.857

10.  Two Active Site Divalent Ions in the Crystal Structure of the Hammerhead Ribozyme Bound to a Transition State Analogue.

Authors:  Aamir Mir; Barbara L Golden
Journal:  Biochemistry       Date:  2016-01-19       Impact factor: 3.162
  10 in total

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