Condensation of chromatin in situ by cation-dependent charge shielding and aggregation.

Binding of di- or polyvalent cations is necessary to maintain the chromatin in isolated nuclei and metaphase chromosomes in a condensed state. We here show that the native, porous structure of the heterochromatin in the interphase nucleus requires both K(+) and Mg(2+) in concentrations, which are known to support transcription in isolated nuclei, thus providing a functional state of the chromatin. When these cations are acting separately, the chromatin is more condensed by Mg(2+) and decondensed by K(+). Comparison with published values of the free electrostatic energy of DNA in chromatin shows that this state of compaction is the result of a balance between the different aggregative properties of K(+) and Mg(2+) at a high degree of charge shielding.