BACKGROUND AND OBJECTIVES: The objectives of this study were to evaluate the clinical utility of MUC2-specific reverse transcriptase-polymerase chain reaction (RT-PCR) in gastric cancer patients and to compare MUC2-specific RT-PCR to carcinoembryonic antigen (CEA)-specific RT-PCR. METHODS: A total of 305 lymph nodes from 28 patients with gastric cancer were histologically examined and analyzed by MUC2 RT-PCR and CEA RT-PCR. RESULTS: MUC2 and CEA were expressed in 17.1 and 7.0% of the 286 histologically negative lymph nodes, respectively. The detection rate of MUC2 was significantly higher than that of CEA (P < 0.01). MUC2 RT-PCR revealed no false positive results in control specimens. Lymph node micrometastases in pT1 gastric cancer were expressed only in perigastric lymph nodes near the tumor and were not detected in tumor less than 30 mm in patients with mucosal cancer. CONCLUSIONS: MUC2-specific RT-PCR is a sensitive and specific method to detect lymph node micrometastases in gastric cancer patients. The distribution of lymph node micrometastases detected by this method may be useful in minimally invasive procedures for gastric cancer, especially pT1 cases. (c) 2004 Wiley-Liss, Inc.
BACKGROUND AND OBJECTIVES: The objectives of this study were to evaluate the clinical utility of MUC2-specific reverse transcriptase-polymerase chain reaction (RT-PCR) in gastric cancerpatients and to compare MUC2-specific RT-PCR to carcinoembryonic antigen (CEA)-specific RT-PCR. METHODS: A total of 305 lymph nodes from 28 patients with gastric cancer were histologically examined and analyzed by MUC2 RT-PCR and CEA RT-PCR. RESULTS:MUC2 and CEA were expressed in 17.1 and 7.0% of the 286 histologically negative lymph nodes, respectively. The detection rate of MUC2 was significantly higher than that of CEA (P < 0.01). MUC2 RT-PCR revealed no false positive results in control specimens. Lymph node micrometastases in pT1gastric cancer were expressed only in perigastric lymph nodes near the tumor and were not detected in tumor less than 30 mm in patients with mucosal cancer. CONCLUSIONS:MUC2-specific RT-PCR is a sensitive and specific method to detect lymph node micrometastases in gastric cancerpatients. The distribution of lymph node micrometastases detected by this method may be useful in minimally invasive procedures for gastric cancer, especially pT1 cases. (c) 2004 Wiley-Liss, Inc.
Authors: Peter Scheunemann; Nikolas H Stoecklein; Kai Hermann; Alexander Rehders; Claus F Eisenberger; Wolfram T Knoefel; Stefan B Hosch Journal: Langenbecks Arch Surg Date: 2008-07-15 Impact factor: 3.445
Authors: Ben Lawrence; Barton Kenney; Bernhard Svejda; Simon Schimmack; Daniele Alaimo; Andrea Barbieri; Jaroslaw Jedrych; Mark Kidd; Irvin Modlin Journal: BMC Res Notes Date: 2012-06-21