OBJECTIVE: Abnormal inflammatory responses are implicated in the pathogenesis of neonatal disease. This study aimed to describe the neonatal cytokine response using an in vitro model of stimulated cord blood. METHODS: Cord blood samples (n = 12) were incubated in RPMI 1640 medium with and without lipopolysaccharide. Concentrations of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-8, interferon (IFN)-gamma and IL-10 were determined by multiplex immunoassay at 0, 1, 3, 6 and 24 hours of incubation. The difference between stimulated and control response was defined as the potential secretory capacity (mean +/- S.E.M.; pg/million white cells). Analysis included a Kruskal-Wallis test and post-hoc Mann-Whitney U test. RESULTS: All cytokine capacities increased rapidly by 1 hour (p<0.001), except IL-10 (p=0.04). TNF-alpha peaked between 3-6 hours (1581 +/- 377 pg/million WC), declining by 24 hours. Similarly, IFN-gamma peaked at 3 hours. Capacity ascended throughout the incubation period for IL-6, IL-8 (631 +/- 75 pg/million WC) and IL-10 (311 +/- 37 pg/million WC). Overall, IFN-gamma capacity was lowest (72 +/- 10 pg/million WC) and IL-6 capacity was greatest (61489 +/- 7059 pg/million WC). CONCLUSION: The neonatal inflammatory response is chronologically similar to that determined in adults. Immature neonatal T-cell function may account for the lower IFN-gamma production. These results may expand our knowledge of neonatal disease, etiology and management.
OBJECTIVE: Abnormal inflammatory responses are implicated in the pathogenesis of neonatal disease. This study aimed to describe the neonatal cytokine response using an in vitro model of stimulated cord blood. METHODS: Cord blood samples (n = 12) were incubated in RPMI 1640 medium with and without lipopolysaccharide. Concentrations of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-8, interferon (IFN)-gamma and IL-10 were determined by multiplex immunoassay at 0, 1, 3, 6 and 24 hours of incubation. The difference between stimulated and control response was defined as the potential secretory capacity (mean +/- S.E.M.; pg/million white cells). Analysis included a Kruskal-Wallis test and post-hoc Mann-Whitney U test. RESULTS: All cytokine capacities increased rapidly by 1 hour (p<0.001), except IL-10 (p=0.04). TNF-alpha peaked between 3-6 hours (1581 +/- 377 pg/million WC), declining by 24 hours. Similarly, IFN-gamma peaked at 3 hours. Capacity ascended throughout the incubation period for IL-6, IL-8 (631 +/- 75 pg/million WC) and IL-10 (311 +/- 37 pg/million WC). Overall, IFN-gamma capacity was lowest (72 +/- 10 pg/million WC) and IL-6 capacity was greatest (61489 +/- 7059 pg/million WC). CONCLUSION: The neonatal inflammatory response is chronologically similar to that determined in adults. Immature neonatal T-cell function may account for the lower IFN-gamma production. These results may expand our knowledge of neonatal disease, etiology and management.
Authors: Brittany Mathias; Juan C Mira; Jonathan P Rehfuss; Jaimar C Rincon; Ricardo Ungaro; Dina C Nacionales; M Cecilia Lopez; Henry V Baker; Lyle L Moldawer; Shawn D Larson Journal: Shock Date: 2017-05 Impact factor: 3.454
Authors: Lee A Denson; Scott A McDonald; Abhik Das; Diana E Schendel; Kristin Skogstrand; David M Hougaard; Seetha Shankaran; Rosemary D Higgins; Waldemar A Carlo; Richard A Ehrenkranz Journal: Am J Perinatol Date: 2016-07-25 Impact factor: 1.862