[Molecular markers and modern phylogenetics of mammals].

Application of modern molecular techniques such as molecular cloning, sequences and polymerase chain reaction of DNA resulted in the increasing of resolution of the phylogenetic analysis and enhanced the role of molecular markers in the evolutionary and taxonomic studies. However, certain properties of the molecular markers are to be taken into consideration when results of the molecular phylogenetic analyses are discussed. This survey reviews the advantages and shortages of different molecular markers (mtDNA and nDNA genes, satellite sequences, long and short retroposons) at the various taxonomic levels. The most part of new phylogenetic reconstructions are established on the results of mtDNA analysis and must be interpreted cautiously because of non-mendelian inheritance of mitochondrial genome. The extremely rapid rate of nucleotide change in mtDNA as compared with nDNA reinforces the saturation in nucleotide sequence and screens the phylogenetic signal. The analysis of nuclear genomes in constrained by that only truly orthologous genes are suitable for the phylogeny reconstruction. So there is a problem to distinguish genes from pseudogenes. Besides, there are some general problems of gene reconstruction such as nucleotide and amino acid compositional shift, long branch attraction and the choice of outgroup. Short interspersed nuclear elements (SINEs) may provide the most valuable phylogenetic information. The markers of multilocus DNA analysis (RAPD-PCR, IS-PCR, RELP, ISSR-PCR), their advantages and shortages are also discussed. A brief survey of the recent studies of molecular phylogeny of mammals for the period of about ten years is presented. The results based on the combined analysis of the mitochondrial and nuclear genes reject the reliability of some previously recognized supraordinal Eutherian taxa in favour of independent range of four main super-order clades: Afrotheria, Xenarthra, Euarchontoglires, and Laurasiatheria. Within these clades, monophyly of some of traditionally recognized orders was proved by molecular data. The recent advances of molecular phylogenetics are very encouraging. However, its future developments are full off serious difficulties. The problem of accumulation of the data turns into the problem of their correct analysis that is more difficult from the methodological point of view. The careful analysis of the conformities and contradictions between different data sets and looking for congruent conclusions deduced from different characters are the most fruitful way of further phylogenetic development.