| Literature DB >> 15488759 |
David Gius1, Hengmi Cui, C Matthew Bradbury, John Cook, Deedee K Smart, Shuping Zhao, Lynn Young, Sheri A Brandenburg, Yali Hu, Kheem S Bisht, Allen S Ho, David Mattson, Lunching Sun, Peter J Munson, Eric Y Chuang, James B Mitchell, Andrew P Feinberg.
Abstract
We tested the hypothesis that the effects on gene expression of altered DNA methylation by 5-aza-2'-deoxycytidine (5-aza-CdR) and genetic (DNMT knockout) manipulation of DNA are similar, and distinct from Trichostatin A (TSA)-induced chromatin decondensation. Surprisingly, the effects of 5-aza-CdR were more similar to those of TSA than to DNMT1, DNMT3B, or double DNMT somatic cell knockout. Furthermore, the effects of 5-aza-CdR were similar at one and five days exposure, suggesting active demethylation or direct influence of both drugs on the stability of methylation and/or chromatin marks. Agents that induce gene activation through hypomethylation may have unintended consequences, since nearly as many genes were downregulated as upregulated after demethylation. In addition, a 75 kb cluster of metallothionein genes was coordinately regulated.Entities:
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Year: 2004 PMID： 15488759 DOI： 10.1016/j.ccr.2004.08.029
Source DB: PubMed Journal: Cancer Cell ISSN： 1535-6108 Impact factor: 31.743