Literature DB >> 15488189

Purification and functional analysis of Ric-8A: a guanine nucleotide exchange factor for G-protein alpha subunits.

Gregory G Tall1, Alfred G Gilman.   

Abstract

Ric-8A (synembryn) has been shown to accelerate the in vitro guanine nucleotide exchange activities of most G-protein alpha subunits (with the exception of Galphas). Methods are presented in this article for the purification of Ric-8A and functional analysis of the effects Ric-8A has on G-protein alpha subunit guanine nucleotide-binding activities. The use of Ric-8A to prepare GTPgammaS-Galpha and nucleotide-free Galpha (in complex with Ric-8A) is described.

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Year:  2004        PMID： 15488189     DOI： 10.1016/S0076-6879(04)90023-7

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  14 in total

1.  Structure of Galpha(i1) bound to a GDP-selective peptide provides insight into guanine nucleotide exchange.

Authors:  Christopher A Johnston; Francis S Willard; Mark R Jezyk; Zoey Fredericks; Erik T Bodor; Miller B Jones; Rainer Blaesius; Val J Watts; T Kendall Harden; John Sondek; J Kevin Ramer; David P Siderovski
Journal:  Structure       Date:  2005-07       Impact factor: 5.006

2.  Molecular chaperoning function of Ric-8 is to fold nascent heterotrimeric G protein α subunits.

Authors:  Puiyee Chan; Celestine J Thomas; Stephen R Sprang; Gregory G Tall
Journal:  Proc Natl Acad Sci U S A       Date:  2013-02-19       Impact factor: 11.205

3.  Dual phosphorylation of Ric-8A enhances its ability to mediate G protein α subunit folding and to stimulate guanine nucleotide exchange.

Authors:  Makaía M Papasergi-Scott; Hannah M Stoveken; Lauren MacConnachie; Pui-Yee Chan; Meital Gabay; Dorothy Wong; Robert S Freeman; Asim A Beg; Gregory G Tall
Journal:  Sci Signal       Date:  2018-05-29       Impact factor: 8.192

4.  Activation of Phospholipase C β by Gβγ and Gαq Involves C-Terminal Rearrangement to Release Autoinhibition.

Authors:  Isaac J Fisher; Meredith L Jenkins; Gregory G Tall; John E Burke; Alan V Smrcka
Journal:  Structure       Date:  2020-05-12       Impact factor: 5.006

5.  Purification of heterotrimeric G protein alpha subunits by GST-Ric-8 association: primary characterization of purified G alpha(olf).

Authors:  PuiYee Chan; Meital Gabay; Forrest A Wright; Wei Kan; Sukru S Oner; Stephen M Lanier; Alan V Smrcka; Joe B Blumer; Gregory G Tall
Journal:  J Biol Chem       Date:  2010-11-29       Impact factor: 5.157

6.  Activation of the regulator of G protein signaling 14-Gαi1-GDP signaling complex is regulated by resistance to inhibitors of cholinesterase-8A.

Authors:  Christopher P Vellano; Feng-Jue Shu; Suneela Ramineni; Cindee K Yates; Gregory G Tall; John R Hepler
Journal:  Biochemistry       Date:  2011-01-11       Impact factor: 3.162

7.  Ric-8B is a GTP-dependent G protein alphas guanine nucleotide exchange factor.

Authors:  PuiYee Chan; Meital Gabay; Forrest A Wright; Gregory G Tall
Journal:  J Biol Chem       Date:  2011-04-05       Impact factor: 5.157

8.  Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization.

Authors:  Limin Wang; Dagang Guo; Bowen Xing; J Jillian Zhang; Hong-Bing Shu; Lin Guo; Xin-Yun Huang
Journal:  J Biol Chem       Date:  2011-07-19       Impact factor: 5.157

9.  Atypical protein kinase Cλ is critical for growth factor receptor-induced dorsal ruffle turnover and cell migration.

Authors:  Bowen Xing; Limin Wang; Dagang Guo; Jianyun Huang; Cedric Espenel; Geri Kreitzer; J Jillian Zhang; Lin Guo; Xin-Yun Huang
Journal:  J Biol Chem       Date:  2013-10-03       Impact factor: 5.157

10.  Production of Phosphorylated Ric-8A proteins using protein kinase CK2.

Authors:  Wenxi Yu; Maiya Yu; Makaía M Papasergi-Scott; Gregory G Tall
Journal:  Protein Expr Purif       Date:  2018-10-02       Impact factor: 1.650
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