BACKGROUND: Prostate cancer is the most frequently diagnosed neoplasm and the second leading cause of cancer-related mortality in men. Although the incidence of prostate cancer increases with age, the link between aging and prostate cancer is poorly understood. METHODS: Affymetrix oligonucleotide microarrays were used to analyze the mRNA expression levels in the dorsolateral prostates from 6- and 18-month-old ACI rats. Real-time RT-PCR and immunohistochemistry was performed to validate microarray data in a select set of genes. RESULTS: Microarray analysis revealed changes in gene expression associated with inflammation, oxidative stress, tissue remodeling, and energy metabolism. Most of these changes have been related to increased proliferative status of the prostate, anti-apoptosis, activated stroma, and alteration of the energy metabolism. CONCLUSIONS: Age-associated alterations in the gene expression profile may put the aging prostate in risk for the initiation, promotion, and progression of neoplastic transformation in both our animal model and humans. (c) 2004 Wiley-Liss, Inc.
BACKGROUND:Prostate cancer is the most frequently diagnosed neoplasm and the second leading cause of cancer-related mortality in men. Although the incidence of prostate cancer increases with age, the link between aging and prostate cancer is poorly understood. METHODS: Affymetrix oligonucleotide microarrays were used to analyze the mRNA expression levels in the dorsolateral prostates from 6- and 18-month-old ACI rats. Real-time RT-PCR and immunohistochemistry was performed to validate microarray data in a select set of genes. RESULTS: Microarray analysis revealed changes in gene expression associated with inflammation, oxidative stress, tissue remodeling, and energy metabolism. Most of these changes have been related to increased proliferative status of the prostate, anti-apoptosis, activated stroma, and alteration of the energy metabolism. CONCLUSIONS: Age-associated alterations in the gene expression profile may put the aging prostate in risk for the initiation, promotion, and progression of neoplastic transformation in both our animal model and humans. (c) 2004 Wiley-Liss, Inc.
Authors: Silvana G P Campos; Bianca F Gonçalves; Wellerson R Scarano; Lara S Corradi; Fernanda C A Santos; Ana M G Custodio; Patricia S L Vilamaior; Rejane M Góes; Sebastião R Taboga Journal: Int J Exp Pathol Date: 2010-10 Impact factor: 1.925
Authors: John P Richie; Arunangshu Das; Ana M Calcagnotto; Cesar A Aliaga; Karam El-Bayoumy Journal: Exp Gerontol Date: 2011-12-24 Impact factor: 4.032
Authors: Daniella Bianchi-Frias; Funda Vakar-Lopez; Ilsa M Coleman; Stephen R Plymate; May J Reed; Peter S Nelson Journal: PLoS One Date: 2010-09-01 Impact factor: 3.240
Authors: Arunangshu Das; James D Bortner; Cesar A Aliaga; Aaron Baker; Anne Stanley; Bruce A Stanley; Matthew Kaag; John P Richie; Karam El-Bayoumy Journal: Prostate Date: 2012-08-21 Impact factor: 4.104