Literature DB >> 15483385

Improving the expression of mini-proinsulin in Pichia pastoris.

José M País-Chanfrau1, Yuneski García, Lisandra Licor, Vladimir Besada, Lila Castellanos-Serra, Cecilia I Cabello, Lester Hernández, Manuel Mansur, Liuba Plana, Abdel Hidalgo, Yanet Támbara, María del C Abrahantes-Pérez, Yoandris del Toro, Jorge Valdés, Eduardo Martínez.   

Abstract

Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 degrees C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l(-1) in the culture supernatant after 160 h of growth.

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Year:  2004        PMID： 15483385     DOI： 10.1023/B:BILE.0000044871.80632.f9

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  8 in total

1.  Buried Kex2 Sites in Glargine Precursor Aggregates Prevent Its Intracellular Processing in Pichia pastoris Muts Strains and the Effect of Methanol-Feeding Strategy and Induction Temperature on Glargine Precursor Production Parameters.

Authors:  Abel Caballero-Pérez; José María Viader-Salvadó; Ana Lucía Herrera-Estala; José Antonio Fuentes-Garibay; Martha Guerrero-Olazarán
Journal:  Appl Biochem Biotechnol       Date:  2021-04-30       Impact factor: 2.926

2.  Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pastoris.

Authors:  Jinjia Wang; Xiaolong Wang; Lei Shi; Yuanxing Zhang; Xiangshan Zhou; Menghao Cai
Journal:  J Ind Microbiol Biotechnol       Date:  2017-12-12       Impact factor: 3.346

3.  Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.

Authors:  Chandrasekhar Gurramkonda; Sulena Polez; Natasa Skoko; Ahmad Adnan; Thomas Gäbel; Dipti Chugh; Sathyamangalam Swaminathan; Navin Khanna; Sergio Tisminetzky; Ursula Rinas
Journal:  Microb Cell Fact       Date:  2010-05-12       Impact factor: 5.328

4.  A Simplified and Efficient Process for Insulin Production in Pichia pastoris.

Authors:  Sulena Polez; Domenico Origi; Sotir Zahariev; Corrado Guarnaccia; Sergio G Tisminetzky; Nataša Skoko; Marco Baralle
Journal:  PLoS One       Date:  2016-12-01       Impact factor: 3.240

5.  Production of human pro-relaxin H2 in the yeast Pichia pastoris.

Authors:  D Cimini; K Della Corte; R Finamore; L Andreozzi; A Stellavato; A V A Pirozzi; F Ferrara; R Formisano; M De Rosa; M Chino; L Lista; A Lombardi; V Pavone; C Schiraldi
Journal:  BMC Biotechnol       Date:  2017-01-14       Impact factor: 2.563

6.  Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.

Authors:  Jinjia Wang; Xiaolong Wang; Lei Shi; Fei Qi; Ping Zhang; Yuanxing Zhang; Xiangshan Zhou; Zhiwei Song; Menghao Cai
Journal:  Sci Rep       Date:  2017-02-02       Impact factor: 4.379

7.  High Expression of Methylotrophic Yeast-Derived Recombinant Human Erythropoietin in a pH-Controlled Batch System.

Authors:  Ahmad Maleki; Farzin Roohvand; Hosnieh Tajerzadeh; Hossein Khanahmad; Maryam B Nobari; Ahmad Beiruti; Abdolhossein Rouholamini Najafabadi
Journal:  Avicenna J Med Biotechnol       Date:  2010-10

8.  Fine-Tuned Enzymatic Hydrolysis of Organosolv Pretreated Forest Materials for the Efficient Production of Cellobiose.

Authors:  Anthi Karnaouri; Evangelos Topakas; Leonidas Matsakas; Ulrika Rova; Paul Christakopoulos
Journal:  Front Chem       Date:  2018-04-19       Impact factor: 5.221
  8 in total

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