Analysis of GM3-Gg3 interaction using clustered gycoconjugate models constructed from glycolipid monolayers and artificial glycoconjugate polymers.

Carbohydrate-carbohydrate interactions between clustered GM3 on the Langmuir monolayer and clustered Gg3 trisaccharide along a polystyrene chain were investigated using surface pressure-area (pi-A) isotherms and surface plasmon resonance (SPR). The pi-A isotherm of the GM3 monolayer was expanded substantially and specifically by the Gg3-trisaccharide-bearing glycoconjugate polystyrene [PN(Gg3)] even at 10(-12) M. The PN(Gg3)-induced expansion of the GM3 monolayer required no calcium ion, and the expansion was strongly inhibited in the presence of urea and acetamido sugars. SPR studies of the GM3-Gg3 interaction were carried out to estimate the affinity constant and specificity of the interaction quantitatively. PN(Gg3) was adsorbed onto the GM3 monolayer strongly and specifically with an apparent affinity constant of Ka = 2.5 x 10(6) M(-1). The mechanism of the GM3-Gg3 interaction was discussed on the basis of the relationship between affinity and structure. We found that the NHAc groups of N -acetylneuraminic acid in GM3 and of GalNAc in Gg3 play an important role in the GM3-Gg3 interaction and that PN(Gg3) recognizes not only some specified portions of GM3 but also the trisaccharide as a whole. Published in 2003.