Literature DB >> 15482423

Detection of koi herpesvirus in common carp, Cyprinus carpio L., by loop-mediated isothermal amplification.

I Gunimaladevi1, T Kono, M N Venugopal, M Sakai.   

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for koi herpes virus (KHV) detection in common carp was designed. A set of four primers, two inner and two outer, were designed based on the sequence of the thymidine kinase (tk) gene of KHV. Time and temperature conditions for detection of KHV were optimized for 60 min at 65 degrees C. The detection limit using LAMP was found to be similar to that by polymerase chain reaction. In this study, we have developed a highly sensitive and rapid diagnostic procedure for detection of KHV infection in common carp.

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Year:  2004        PMID： 15482423     DOI： 10.1111/j.1365-2761.2004.00578.x

Source DB:  PubMed          Journal:  J Fish Dis        ISSN： 0140-7775            Impact factor:   2.767


  17 in total

1.  Koi herpes virus: a review and risk assessment of Indian aquaculture.

Authors:  Gaurav Rathore; Gokhlesh Kumar; T Raja Swaminathan; P Swain
Journal:  Indian J Virol       Date:  2012-09-06

2.  Rapid Detection of Cyprinid Herpesvirus 3 in Latently Infected Koi by Recombinase Polymerase Amplification.

Authors:  Meagan A Prescott; Aimee N Reed; Ling Jin; Manoj K Pastey
Journal:  J Aquat Anim Health       Date:  2016-09       Impact factor: 1.625

Review 3.  CyHV-3: the third cyprinid herpesvirus.

Authors:  Michael Gotesman; Julia Kattlun; Sven M Bergmann; Mansour El-Matbouli
Journal:  Dis Aquat Organ       Date:  2013-07-22       Impact factor: 1.802

4.  Cloning of the koi herpesvirus genome as an infectious bacterial artificial chromosome demonstrates that disruption of the thymidine kinase locus induces partial attenuation in Cyprinus carpio koi.

Authors:  B Costes; G Fournier; B Michel; C Delforge; V Stalin Raj; B Dewals; L Gillet; P Drion; A Body; F Schynts; F Lieffrig; A Vanderplasschen
Journal:  J Virol       Date:  2008-03-12       Impact factor: 5.103

Review 5.  Characterization of a novel virus causing a lethal disease in carp and koi.

Authors:  Maya Ilouze; Arnon Dishon; Moshe Kotler
Journal:  Microbiol Mol Biol Rev       Date:  2006-03       Impact factor: 11.056

6.  Specific, simple and rapid detection of porcine circovirus type 2 using the loop-mediated isothermal amplification method.

Authors:  Kai Zhao; Wei Shi; Fangting Han; Yan Xu; Lianlong Zhu; Yong Zou; Xiao Wu; Hong Zhu; Furong Tan; Shiru Tao; Xueming Tang
Journal:  Virol J       Date:  2011-03-18       Impact factor: 4.099

7.  An inexpensive and rapid diagnostic method of Koi Herpesvirus (KHV) infection by loop-mediated isothermal amplification.

Authors:  Hatem Soliman; Mansour El-Matbouli
Journal:  Virol J       Date:  2005-10-17       Impact factor: 4.099

8.  Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis.

Authors:  Herve Bercovier; Yolanta Fishman; Ronen Nahary; Sharon Sinai; Amir Zlotkin; Marina Eyngor; Oren Gilad; Avi Eldar; Ronald P Hedrick
Journal:  BMC Microbiol       Date:  2005-03-17       Impact factor: 3.605

9.  A loop-mediated isothermal amplification assay for rapid detection of cyprinid herpesvirus 2 in gibel carp (Carassius auratus gibelio).

Authors:  Hui Zhang; Lingbing Zeng; Yuding Fan; Yong Zhou; Jin Xu; Jie Ma
Journal:  ScientificWorldJournal       Date:  2014-01-19

Review 10.  A new age in AquaMedicine: unconventional approach in studying aquatic diseases.

Authors:  Michael Gotesman; Simon Menanteau-Ledouble; Mona Saleh; Sven M Bergmann; Mansour El-Matbouli
Journal:  BMC Vet Res       Date:  2018-06-08       Impact factor: 2.741
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