Literature DB >> 1548242

Differential depolymerization mechanisms of pectate lyases secreted by Erwinia chrysanthemi EC16.

J F Preston1, J D Rice, L O Ingram, N T Keen.   

Abstract

The four pectate lyases (EC 4.2.2.2) secreted by Erwinia chrysanthemi EC16 have been individually produced as recombinant enzymes in Escherichia coli. Oligogalacturonates formed from polygalacturonic acid during reactions catalyzed by each enzyme have been determined by high-performance liquid chromatography analysis. PLa catalyzes the formation of a series of oligomers ranging from dimer to dodecamer through a random endolytic depolarization mechanism. PLb and PLc are trimer- and tetramer-generating enzymes with an identical combination of endolytic and exolytic mechanisms. PLe catalyzes a nonrandom endolytic depolymerization with the formation of dimer as the predominant product. The pectate lyases secreted by E. chrysanthemi EC16 represent a battery of enzymes with three distinct approaches to the depolymerization of plant cell walls.

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Year:  1992        PMID： 1548242      PMCID： PMC205812          DOI： 10.1128/jb.174.6.2039-2042.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  Resolution of four pectate lyase structural genes of Erwinia chrysanthemi (EC16) and characterization of the enzymes produced in Escherichia coli.

Authors:  F Barras; K K Thurn; A K Chatterjee
Journal:  Mol Gen Genet       Date:  1987-09

2.  Structure and organization of the pel genes from Erwinia chrysanthemi EC16.

Authors:  S J Tamaki; S Gold; M Robeson; S Manulis; N T Keen
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

3.  Characterization of Erwinia chrysanthemi extracellular proteases: cloning and expression of the protease genes in Escherichia coli.

Authors:  C Wandersman; P Delepelaire; S Letoffe; M Schwartz
Journal:  J Bacteriol       Date:  1987-11       Impact factor: 3.490

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli.

Authors:  B Witholt; M Boekhout; M Brock; J Kingma; H V Heerikhuizen; L D Leij
Journal:  Anal Biochem       Date:  1976-07       Impact factor: 3.365

Review 6.  Genetics of Erwinia species.

Authors:  A K Chatterjee; M P Starr
Journal:  Annu Rev Microbiol       Date:  1980       Impact factor: 15.500

7.  Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16.

Authors:  G S Dahler; F Barras; N T Keen
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

8.  Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthemi EC16.

Authors:  S Y He; A Collmer
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

9.  Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and their high-level expression in Escherichia coli.

Authors:  N T Keen; S Tamaki
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490
  9 in total
  20 in total

Review 1.  Structure and function of pectic enzymes: virulence factors of plant pathogens.

Authors:  S R Herron; J A Benen; R D Scavetta; J Visser; F Jurnak
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-01       Impact factor: 11.205

2.  The Three-Dimensional Structure of Pectate Lyase E, a Plant Virulence Factor from Erwinia chrysanthemi.

Authors:  S. E. Lietzke; M. D. Yoder; N. T. Keen; F. Jurnak
Journal:  Plant Physiol       Date:  1994-11       Impact factor: 8.340

3.  The Refined Three-Dimensional Structure of Pectate Lyase E from Erwinia chrysanthemi at 2.2 A Resolution.

Authors:  S. E. Lietzke; R. D. Scavetta; M. D. Yoder; F. Jurnak
Journal:  Plant Physiol       Date:  1996-05       Impact factor: 8.340

4.  Erwinia chrysanthemi EC16 Produces a Second Set of Plant-Inducible Pectate Lyase Isozymes.

Authors:  S Kelemu; A Collmer
Journal:  Appl Environ Microbiol       Date:  1993-06       Impact factor: 4.792

5.  Biochemical properties of pectate lyases produced by three different Bacillus strains isolated from fermenting cocoa beans and characterization of their cloned genes.

Authors:  Honoré G Ouattara; Sylvie Reverchon; Sébastien L Niamke; William Nasser
Journal:  Appl Environ Microbiol       Date:  2010-06-11       Impact factor: 4.792

6.  Functional implications of structure-based sequence alignment of proteins in the extracellular pectate lyase superfamily.

Authors:  B Henrissat; S E Heffron; M D Yoder; S E Lietzke; F Jurnak
Journal:  Plant Physiol       Date:  1995-03       Impact factor: 8.340

7.  The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937.

Authors:  V E Shevchik; G Condemine; J Robert-Baudouy; N Hugouvieux-Cotte-Pattat
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

8.  Modes of action of five different endopectate lyases from Erwinia chrysanthemi 3937.

Authors:  C Roy; H Kester; J Visser; V Shevchik; N Hugouvieux-Cotte-Pattat; J Robert-Baudouy; J Benen
Journal:  J Bacteriol       Date:  1999-06       Impact factor: 3.490

Review 9.  Detection of and response to signals involved in host-microbe interactions by plant-associated bacteria.

Authors:  Anja Brencic; Stephen C Winans
Journal:  Microbiol Mol Biol Rev       Date:  2005-03       Impact factor: 11.056

10.  The Refined Three-Dimensional Structure of Pectate Lyase C from Erwinia chrysanthemi at 2.2 Angstrom Resolution (Implications for an Enzymatic Mechanism).

Authors:  M. D. Yoder; F. Jurnak
Journal:  Plant Physiol       Date:  1995-02       Impact factor: 8.340
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