PURPOSE: We have previously demonstrated that conditioned medium from bacteria, some of which were isolated from the colon of cancer patients, stimulate cancer cell invasion in vitro through a 13-mer beta-casein-derived peptide. Since invasion signalling pathways are coordinated by the balance between protein kinases and phosphatases, we investigated the effect of conditioned medium from bacteria on the overall cellular tyrosine phosphorylation. METHODS: The tyrosine phosphorylation level of HCT-8/E11 human colon cancer cells treated with the pro-invasive conditioned medium of Listeria, prepared on top of collagen type I gels (CM(Coll) Listeria/TSB), were analysed by means of immunoprecipitation and Western blot, with specific anti-phosphotyrosine antibodies. RESULTS: We demonstrated that CM(Coll) Listeria/TSB increases the tyrosine phosphorylation level of ErbB2 and ErbB3, members of the epidermal growth factor receptor (EGFR) family, and the association between ErbB3 and the phosphatidylinositol 3-kinase (PI3K) regulatory subunit (p85alpha). CM(Coll) Listeria/TSB-stimulated ErbB3 tyrosine phosphorylation and cancer cell invasion were independent from EGFR expression and activity but dependent on ErbB2 activity. CONCLUSIONS: The interaction between Listeria and collagen type I produces, next to the 13-mer peptide, at least another pro-invasive factor that signals via ErbB2/ErbB3 heterodimers.
PURPOSE: We have previously demonstrated that conditioned medium from bacteria, some of which were isolated from the colon of cancerpatients, stimulate cancer cell invasion in vitro through a 13-mer beta-casein-derived peptide. Since invasion signalling pathways are coordinated by the balance between protein kinases and phosphatases, we investigated the effect of conditioned medium from bacteria on the overall cellular tyrosine phosphorylation. METHODS: The tyrosine phosphorylation level of HCT-8/E11 humancolon cancer cells treated with the pro-invasive conditioned medium of Listeria, prepared on top of collagen type I gels (CM(Coll) Listeria/TSB), were analysed by means of immunoprecipitation and Western blot, with specific anti-phosphotyrosine antibodies. RESULTS: We demonstrated that CM(Coll) Listeria/TSB increases the tyrosine phosphorylation level of ErbB2 and ErbB3, members of the epidermal growth factor receptor (EGFR) family, and the association between ErbB3 and the phosphatidylinositol 3-kinase (PI3K) regulatory subunit (p85alpha). CM(Coll) Listeria/TSB-stimulated ErbB3 tyrosine phosphorylation and cancer cell invasion were independent from EGFR expression and activity but dependent on ErbB2 activity. CONCLUSIONS: The interaction between Listeria and collagen type I produces, next to the 13-mer peptide, at least another pro-invasive factor that signals via ErbB2/ErbB3 heterodimers.
Authors: M Barbier; S Attoub; R Calvez; M Laffargue; A Jarry; M Mareel; F Altruda; C Gespach; D Wu; B Lu; E Hirsch; M P Wymann Journal: Nature Date: 2001-10-25 Impact factor: 49.962
Authors: Alberto Bardelli; D Williams Parsons; Natalie Silliman; Janine Ptak; Steve Szabo; Saurabh Saha; Sanford Markowitz; James K V Willson; Giovanni Parmigiani; Kenneth W Kinzler; Bert Vogelstein; Victor E Velculescu Journal: Science Date: 2003-05-09 Impact factor: 47.728
Authors: S Kado; K Uchida; H Funabashi; S Iwata; Y Nagata; M Ando; M Onoue; Y Matsuoka; M Ohwaki; M Morotomi Journal: Cancer Res Date: 2001-03-15 Impact factor: 12.701
Authors: Maria José Oliveira; Jozef Van Damme; Tineke Lauwaet; Veerle De Corte; Georges De Bruyne; Gerda Verschraegen; Mario Vaneechoutte; Marc Goethals; Mohammad Reza Ahmadian; Oliver Müller; Joël Vandekerckhove; Marc Mareel; Ancy Leroy Journal: EMBO J Date: 2003-11-17 Impact factor: 11.598