Literature DB >> 1547951

A modified kanamycin-resistance cassette to facilitate two-codon insertion mutagenesis.

W J Chen1, L Gross, K E Joho, W T McAllister.   

Abstract

A kanamycin-resistance (KmR) cassette was modified to allow its introduction as a blunt-ended DNA fragment into target plasmids. Subsequent excision of the KmR cassette with ApaI and religation of the plasmid leaves behind six base pairs (5'-GGGCCC) that result in the insertion of two amino acids in the target protein.

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Year:  1992        PMID： 1547951     DOI： 10.1016/0378-1119(92)90617-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  5 in total

1.  Genetic evidence that the bacteriophage phi X174 lysis protein inhibits cell wall synthesis.

Authors:  T G Bernhardt; W D Roof; R Young
Journal:  Proc Natl Acad Sci U S A       Date:  2000-04-11       Impact factor: 11.205

2.  Point mutations in a peptidoglycan biosynthesis gene cause competence induction in Haemophilus influenzae.

Authors:  C Ma; R J Redfield
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

3.  Stimulation of glucose catabolism in Escherichia coli by a potential futile cycle.

Authors:  R Patnaik; W D Roof; R F Young; J C Liao
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

4.  Control of gluconeogenic growth by pps and pck in Escherichia coli.

Authors:  Y P Chao; R Patnaik; W D Roof; R F Young; J C Liao
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490

5.  Alteration of growth yield by overexpression of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase in Escherichia coli.

Authors:  Y P Chao; J C Liao
Journal:  Appl Environ Microbiol       Date:  1993-12       Impact factor: 4.792
  5 in total

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