OBJECTIVE: In this study, we postulated the beneficial role of oral alanyl-glutamine, a more stable glutamine derivative to decrease 5-fluorouracil (5-FU)-induced mucositis in mice. METHODS: We measured different morphologic parameters to assess structural changes over time in the small bowel, including crypt depth, villus height, villus area, mitotic and apoptotic indices at the crypt level using terminal deoxyuridine triphosphate nick end labeling, and hematoxylin-eosin staining of ileal tissue. In addition, we analyzed the effect of different alanyl-glutamine concentrations on animal weight curves after 5-FU treatment. RESULTS: Neither glutamine nor alanyl- glutamine prevented the 5-FU intestinal structural damage or apoptosis in crypt enterocytes at 24 h after 5-FU challenge. However, we found that alanyl-glutamine, but not glutamine, speeds intestinal recovery when compared with 5-FU-treated controls (P < 0.05), predominantly by enhancing mitotic activity and crypt length. CONCLUSION: Our findings provide important data to support clinical studies of oral alanyl-glutamine in 5-FU-induced mucositis.
OBJECTIVE: In this study, we postulated the beneficial role of oral alanyl-glutamine, a more stable glutamine derivative to decrease 5-fluorouracil (5-FU)-induced mucositis in mice. METHODS: We measured different morphologic parameters to assess structural changes over time in the small bowel, including crypt depth, villus height, villus area, mitotic and apoptotic indices at the crypt level using terminal deoxyuridine triphosphate nick end labeling, and hematoxylin-eosin staining of ileal tissue. In addition, we analyzed the effect of different alanyl-glutamine concentrations on animal weight curves after 5-FU treatment. RESULTS: Neither glutamine nor alanyl- glutamine prevented the 5-FU intestinal structural damage or apoptosis in crypt enterocytes at 24 h after 5-FU challenge. However, we found that alanyl-glutamine, but not glutamine, speeds intestinal recovery when compared with 5-FU-treated controls (P < 0.05), predominantly by enhancing mitotic activity and crypt length. CONCLUSION: Our findings provide important data to support clinical studies of oral alanyl-glutamine in 5-FU-induced mucositis.
Authors: Ibraim C Castro; Bruna B Oliveira; Jacek J Slowikowski; Bruna P Coutinho; Francisco Júlio W S Siqueira; Lourrany B Costa; Jesus Emmanuel Sevilleja; Camila A Almeida; Aldo A M Lima; Cirle A Warren; Reinaldo B Oriá; Richard L Guerrant Journal: Nutrition Date: 2012-01-20 Impact factor: 4.008
Authors: Benedito A Carneiro; Jun Fujii; Gerly A C Brito; Cirle Alcantara; Reinaldo B Oriá; Aldo A M Lima; Tom Obrig; Richard L Guerrant Journal: Infect Immun Date: 2006-01 Impact factor: 3.441
Authors: Barbara Vanhoecke; Emma Bateman; Bronwen Mayo; Eline Vanlancker; Andrea Stringer; Daniel Thorpe; Dorothy Keefe Journal: Exp Biol Med (Maywood) Date: 2015-05-12
Authors: Priscilla M Ueno; Reinaldo B Oriá; Elizabeth A Maier; Marjorie Guedes; Orleancio G de Azevedo; David Wu; Tara Willson; Simon P Hogan; Aldo A M Lima; Richard L Guerrant; D Brent Polk; Lee A Denson; Sean R Moore Journal: Am J Physiol Gastrointest Liver Physiol Date: 2011-07-28 Impact factor: 4.052
Authors: Andressa A F L Maciel; Reinaldo B Oriá; Manuel B Braga-Neto; Andréa B Braga; Eunice B Carvalho; Herene B M Lucena; Gerly A C Brito; Richard L Guerrant; Aldo A M Lima Journal: Toxicon Date: 2007-07-31 Impact factor: 3.033
Authors: Bruna P Coutinho; Reinaldo B Oriá; Carlos M G Vieira; Jesus Emmanuel A D Sevilleja; Cirle A Warren; Jamilly G Maciel; Meghan R Thompson; Relana C Pinkerton; Aldo A M Lima; Richard L Guerrant Journal: J Parasitol Date: 2008-12 Impact factor: 1.276
Authors: Manuel B Braga-Neto; Bruna M C Oliveira; Raphael S Rodrigues; Francisco J Noronha; Renata F Leitao; Gerly A C Brito; Aldo A Lima; Richard L Guerrant; Cirle A Warren Journal: Cancer Biol Ther Date: 2012-09-17 Impact factor: 4.742
Authors: Reinaldo B Oriá; Carlos Meton G Vieira; Relana C Pinkerton; Carlos M de Castro Costa; Maria Beatriz Lopes; Isa Hussaini; Weibin Shi; Gerly A C Brito; Aldo A M Lima; Richard L Guerrant Journal: Nutr Res Date: 2006-08 Impact factor: 3.315
Authors: Manuel B Braga-Neto; Cirle A Warren; Reinaldo B Oriá; Manuel S Monteiro; Andressa A S Maciel; Gerly A C Brito; Aldo A M Lima; Richard L Guerrant Journal: Dig Dis Sci Date: 2008-03-06 Impact factor: 3.199