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Literature DB >> 15472343

Tobacco agar, a new medium for differentiating Candida dubliniensis from Candida albicans.

Zia U Khan¹, Suhail Ahmad, Eiman Mokaddas, Rachel Chandy.

Abstract

Isolates of Candida dubliniensis may be misidentified as Candida albicans in microbiological laboratories if only the germ tube and/or the chlamydospore test is used for identification to the species level. In this study, we have evaluated the efficacy of tobacco agar for the differentiation of C. dubliniensis from C. albicans. On this medium at 28 degrees C, all 30 C. dubliniensis isolates produced yellowish-brown colonies with hyphal fringes and abundant chlamydospores, whereas 54 C. albicans isolates formed smooth, white-to-cream-colored colonies with no chlamydospore production. This medium provides a simple tool for presumptive differentiation of C. dubliniensis from C. albicans.

Entities: Chemical Species

Mesh：

Substances：
Culture Media
Agar

Year: 2004 PMID： 15472343 PMCID： PMC522288 DOI： 10.1128/JCM.42.10.4796-4798.2004

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

18 in total

1. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.

Authors: Suhail Ahmad; Zaiba Khan; Abu S Mustafa; Zia U Khan
Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948

2. Use of specialised isolation media for recognition and identification of Candida dubliniensis isolates from HIV-infected patients.

Authors: A Schoofs; F C Odds; R Colebunders; M Ieven; H Goossens
Journal: Eur J Clin Microbiol Infect Dis Date: 1997-04 Impact factor: 3.267

3. Recovery of Candida dubliniensis from non-human immunodeficiency virus-infected patients in Israel.

Authors: I Polacheck; J Strahilevitz; D Sullivan; S Donnelly; I F Salkin; D C Coleman
Journal: J Clin Microbiol Date: 2000-01 Impact factor: 5.948

4. Candida dubliniensis fungemia in a solid organ transplant patient: case report and review of the literature.

Authors: G S Gottlieb; A P Limaye; Y C Chen; W C Van Voorhis
Journal: Med Mycol Date: 2001-12 Impact factor: 4.076

5. Differentiation of Candida dubliniensis from Candida albicans on staib agar and caffeic acid-ferric citrate agar.

Authors: A Al Mosaid; D Sullivan; I F Salkin; D Shanley; D C Coleman
Journal: J Clin Microbiol Date: 2001-01 Impact factor: 5.948

6. Prevalence of Candida dubliniensis isolates in a yeast stock collection.

Authors: F C Odds; L Van Nuffel; G Dams
Journal: J Clin Microbiol Date: 1998-10 Impact factor: 5.948

7. Candida dubliniensis at a university hospital in Saudi Arabia.

Authors: R Fotedar; S S A Al-Hedaithy
Journal: J Clin Microbiol Date: 2003-05 Impact factor: 5.948

8. Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals.

Authors: D J Sullivan; T J Westerneng; K A Haynes; D E Bennett; D C Coleman
Journal: Microbiology Date: 1995-07 Impact factor: 2.777

9. Evaluation of a method for identification of Candida dubliniensis bloodstream isolates.

Authors: Banu Sancak; John H Rex; Victor Paetznick; Enuo Chen; Jose Rodriguez
Journal: J Clin Microbiol Date: 2003-01 Impact factor: 5.948

10. Rapid and unequivocal differentiation of Candida dubliniensis from other Candida species using species-specific DNA probes: comparison with phenotypic identification methods.

Authors: A N B Ellepola; S F Hurst; C M Elie; C J Morrison
Journal: Oral Microbiol Immunol Date: 2003-12

16 in total

1. Prevalence of Candida dubliniensis fungemia in Argentina: identification by a novel multiplex PCR and comparison of different phenotypic methods.

Authors: Maria Eugenia Bosco-Borgeat; Constanza Giselle Taverna; Susana Cordoba; Maria Guillermina Isla; Omar Alejandro Murisengo; Wanda Szusz; Walter Vivot; Graciela Davel
Journal: Mycopathologia Date: 2011-07-13 Impact factor: 2.574

Tobacco agar, a new medium for differentiating Candida dubliniensis from Candida albicans.

1. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.

2. Use of specialised isolation media for recognition and identification of Candida dubliniensis isolates from HIV-infected patients.

3. Recovery of Candida dubliniensis from non-human immunodeficiency virus-infected patients in Israel.

4. Candida dubliniensis fungemia in a solid organ transplant patient: case report and review of the literature.

5. Differentiation of Candida dubliniensis from Candida albicans on staib agar and caffeic acid-ferric citrate agar.

6. Prevalence of Candida dubliniensis isolates in a yeast stock collection.

7. Candida dubliniensis at a university hospital in Saudi Arabia.

8. Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals.

9. Evaluation of a method for identification of Candida dubliniensis bloodstream isolates.

10. Rapid and unequivocal differentiation of Candida dubliniensis from other Candida species using species-specific DNA probes: comparison with phenotypic identification methods.

1. Prevalence of Candida dubliniensis fungemia in Argentina: identification by a novel multiplex PCR and comparison of different phenotypic methods.

2. Smoking, melanization, and cryptococcosis: is there a connection?

3. Chlamydospore formation during hyphal growth in Cryptococcus neoformans.

4. First isolation of Candida dubliniensis from oral cavities of dermatological patients in Nanjing, China.

5. Methods of Candida dubliniensis identification and its occurrence in human clinical material.

6. Hypertonic sabouraud dextrose agar as a substrate for differentiation of Candida dubliniensis.

7. Differentiation of Candida dubliniensis from Candida albicans on rosemary extract agar and oregano extract agar.

8. Epidemiology and antifungal susceptibilities of yeasts causing vulvovaginitis in a teaching hospital.

9. Plant-derived sucrose is a key element in the symbiotic association between Trichoderma virens and maize plants.

10. Onychomycosis incidence in type 2 diabetes mellitus patients.