| Literature DB >> 15470897 |
Sudip Ghosh1, Sheeba Rasheedi, Sheikh Showkat Rahim, Sharmistha Banerjee, Rakesh Kumar Choudhary, Prachee Chakhaiyar, Nasreen Z Ehtesham, Sangita Mukhopadhyay, Seyed E Hasnain.
Abstract
The production of correctly folded protein in Escherichia coli is often challenging because of aggregation of the overexpressed protein into inclusion bodies. Although a number of general and protein-specific techniques are available, their effectiveness varies widely. We report a novel method for enhancing the solubility of overexpressed proteins. Presence of a dipeptide, glycylglycine, in the range of 100 mM to 1 M in the medium was found to significantly enhance the solubility (up to 170-fold) of the expressed proteins. The method has been validated using mycobacterial proteins, resulting in improved solubilization, which were otherwise difficult to express as soluble proteins in E. coli. This method can also be used to enhance the solubility of other heterologous recombinant proteins expressed in a bacterial system.Entities:
Mesh：
Substances：
Year: 2004 PMID： 15470897 DOI： 10.2144/04373ST07
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993