Literature DB >> 1546949

Purification and some properties of alcohol oxidase from alkane-grown Candida tropicalis.

F M Dickinson1, C Wadforth.   

Abstract

Alcohol oxidase was purified to homogeneity from membrane fractions obtained from alkane-grown Candida tropicalis. The enzyme appears to be a dimer of equal-sized subunits of Mr 70000. The purified enzyme is photosensitive and contains flavin-type material which is released by a combination of boiling and proteolytic digestion. The identity of the flavin material is not yet known, but it is not FMN, FAD or riboflavin. The enzyme is most active with dodecan-I-ol, but other long-chain alcohols are also attacked. The enzyme shows a weak, but significant activity towards long-chain aldehydes. Detailed kinetic studies with decan-1-ol as substrate suggest a group-transfer (Ping-Pong)-type mechanism of catalysis.

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Year:  1992        PMID： 1546949      PMCID： PMC1130782          DOI： 10.1042/bj2820325

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Amine oxidases from methylotrophic yeasts.

Authors:  P J Large; G W Haywood
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

2.  Microbial assimilation of methanol. The ethanol- and methanol-oxidizing enzymes of the yeast Candida boidinii.

Authors:  H Sahm; F Wagner
Journal:  Eur J Biochem       Date:  1973-07-02

3.  Aldehyde mutase.

Authors:  K Dalziel; F M Dickinson
Journal:  Nature       Date:  1965-04-17       Impact factor: 49.962

4.  Structure of the covalently bound coenzyme of trimethylamine dehydrogenase. Evidence for a 6-substituted flavin.

Authors:  D J Steenkamp; W McIntire; W C Kenney
Journal:  J Biol Chem       Date:  1978-04-25       Impact factor: 5.157

5.  Microbial oxidation of methanol: properties of crystallized alcohol oxidase from a yeast, Pichia sp.

Authors:  R N Patel; C T Hou; A I Laskin; P Derelanko
Journal:  Arch Biochem Biophys       Date:  1981-09       Impact factor: 4.013

6.  Determination of FMN and FAD by fluorescence titration with apoflavodoxin.

Authors:  S G Mayhew; J H Wassink
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  Specific detection of quinoproteins by redox-cycling staining.

Authors:  M A Paz; R Flückiger; A Boak; H M Kagan; P M Gallop
Journal:  J Biol Chem       Date:  1991-01-15       Impact factor: 5.157

8.  Microbial oxidation of amines. Distribution, purification and properties of two primary-amine oxidases from the yeast Candida boidinii grown on amines as sole nitrogen source.

Authors:  G W Haywood; P J Large
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857

9.  Measurement of protein using bicinchoninic acid.

Authors:  P K Smith; R I Krohn; G T Hermanson; A K Mallia; F H Gartner; M D Provenzano; E K Fujimoto; N M Goeke; B J Olson; D C Klenk
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365

10.  A novel type of covalently bound coenzyme in trimethylamine dehydrogenase.

Authors:  D J Steenkamp; W C Kenney; T P Singer
Journal:  J Biol Chem       Date:  1978-04-25       Impact factor: 5.157
  10 in total
  2 in total

1.  Cloning and characterization of three fatty alcohol oxidase genes from Candida tropicalis strain ATCC 20336.

Authors:  L Dudley Eirich; David L Craft; Lisa Steinberg; Afreen Asif; William H Eschenfeldt; Lucy Stols; Mark I Donnelly; C Ron Wilson
Journal:  Appl Environ Microbiol       Date:  2004-08       Impact factor: 4.792

2.  Molecular characterization and expression of a novel alcohol oxidase from Aspergillus terreus MTCC6324.

Authors:  Mitun Chakraborty; Manish Goel; Somasekhar R Chinnadayyala; Ujjwal Ranjan Dahiya; Siddhartha Sankar Ghosh; Pranab Goswami
Journal:  PLoS One       Date:  2014-04-21       Impact factor: 3.240
  2 in total

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