Literature DB >> 15466588

Cloning and expression of clt genes encoding milk-clotting proteases from Myxococcus xanthus 422.

M Poza1, M Prieto-Alcedo, C Sieiro, T G Villa.   

Abstract

The screening of a gene library of the milk-clotting strain Myxococcus xanthus 422 constructed in Escherichia coli allowed the description of eight positive clones containing 26 open reading frames. Only three of them (cltA, cltB, and cltC) encoded proteins that exhibited intracellular milk-clotting ability in E. coli, Saccharomyces cerevisiae, and Pichia pastoris expression systems.

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Year:  2004        PMID： 15466588      PMCID： PMC522063          DOI： 10.1128/AEM.70.10.6337-6341.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  36 in total

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Journal:  Science       Date:  1985-09-20       Impact factor: 47.728

2.  A novel aspartyl protease allowing KEX2-independent MF alpha propheromone processing in yeast.

Authors:  M Egel-Mitani; H P Flygenring; M T Hansen
Journal:  Yeast       Date:  1990 Mar-Apr       Impact factor: 3.239

3.  Expression of calf prochymosin in Saccharomyces cerevisiae.

Authors:  C G Goff; D T Moir; T Kohno; T C Gravius; R A Smith; E Yamasaki; A Taunton-Rigby
Journal:  Gene       Date:  1984-01       Impact factor: 3.688

4.  Primary structure of Mucor miehei aspartyl protease: evidence for a zymogen intermediate.

Authors:  G L Gray; K Hayenga; D Cullen; L J Wilson; S Norton
Journal:  Gene       Date:  1986       Impact factor: 3.688

5.  Molecular cloning and characterization of double-stranded cDNA coding for bovine chymosin.

Authors:  D Moir; J Mao; J W Schumm; G F Vovis; B L Alford; A Taunton-Rigby
Journal:  Gene       Date:  1982 Jul-Aug       Impact factor: 3.688

6.  Transformation of intact yeast cells treated with alkali cations.

Authors:  H Ito; Y Fukuda; K Murata; A Kimura
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

7.  Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae.

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Journal:  Gene       Date:  1983-09       Impact factor: 3.688

8.  Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin.

Authors:  J A van den Berg; K J van der Laken; A J van Ooyen; T C Renniers; K Rietveld; A Schaap; A J Brake; R J Bishop; K Schultz; D Moyer
Journal:  Biotechnology (N Y)       Date:  1990-02

9.  Enzyme production by recombinant Trichoderma reesei strains.

Authors:  J M Uusitalo; K M Nevalainen; A M Harkki; J K Knowles; M E Penttilä
Journal:  J Biotechnol       Date:  1991-01       Impact factor: 3.307

10.  Synthesis of calf prochymosin (prorennin) in Escherichia coli.

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Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205
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  3 in total

1.  Constitutive expression, purification and characterization of bovine prochymosin in Pichia pastoris GS115.

Authors:  X P Jiang; M L Yin; P Chen; Q Yang
Journal:  World J Microbiol Biotechnol       Date:  2012-02-10       Impact factor: 3.312

2.  Proteins associated with the Myxococcus xanthus extracellular matrix.

Authors:  Patrick D Curtis; James Atwood; Ron Orlando; Lawrence J Shimkets
Journal:  J Bacteriol       Date:  2007-08-31       Impact factor: 3.490

3.  Cloning and expression of an active aspartic proteinase from Mucor circinelloides in Pichia pastoris.

Authors:  Jose Antonio Gama Salgado; Martin Kangwa; Marcelo Fernandez-Lahore
Journal:  BMC Microbiol       Date:  2013-11-09       Impact factor: 3.605
  3 in total

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