| Literature DB >> 15466586 |
Dario De Medici1, Luciana Croci, Elisabetta Suffredini, Laura Toti.
Abstract
The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NV strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in tests on experimentally and naturally contaminated shellfish.Mesh：
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Year: 2004 PMID： 15466586 PMCID： PMC522127 DOI： 10.1128/AEM.70.10.6329-6332.2004
Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792