| Literature DB >> 15463942 |
Caroline Norez1, Ghanshyam D Heda, Timothy Jensen, Ilana Kogan, Lauren K Hughes, Céline Auzanneau, Renaud Dérand, Laurence Bulteau-Pignoux, Canhui Li, Mohabir Ramjeesingh, Hongyu Li, David N Sheppard, Christine E Bear, John R Riordan, Frédéric Becq.
Abstract
Flux studies using either radioisotopes or ion-selective electrodes are a convenient method to assay the function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. Here, we described three different protocols to study the properties, regulation and pharmacology of the CFTR Cl- channel in populations of cells and artificial vesicles. These techniques are widely used to evaluate the function of wild-type and mutant CFTR prior to detailed analyses using the patch-clamp technique. Moreover, they have proved especially valuable in the search for new drugs to treat cystic fibrosis.Entities:
Mesh：
Substances：
Year: 2004 PMID： 15463942 DOI： 10.1016/j.jcf.2004.05.025
Source DB: PubMed Journal: J Cyst Fibros ISSN： 1569-1993 Impact factor: 5.482