| Literature DB >> 15463923 |
Filipa Mendes1, Laurent Doucet, Alexandre Hinzpeter, Claude Férec, Joanna Lipecka, Janine Fritsch, Aleksander Edelman, Huub Jorna, Rob Willemsen, Alice G M Bot, Hugo R De Jonge, Jocelyne Hinnrasky, Nicolas Castillon, Karima Taouil, Edith Puchelle, Deborah Penque, Margarida D Amaral.
Abstract
Studies on CFTR protein expression and localization in native tissues or in primary cultures of human epithelial cells are scarce due to the intrinsic instability of this protein, its low expression in most tissues and also to technical difficulties. However, such data are of the highest importance to understand the pathophysiology of CF. The purpose of this article is to outline several assays for the characterization of primary epithelial cultures and to review different CFTR immunostaining protocols.Entities:
Mesh：
Substances：
Year: 2004 PMID： 15463923 DOI： 10.1016/j.jcf.2004.05.008
Source DB: PubMed Journal: J Cyst Fibros ISSN： 1569-1993 Impact factor: 5.482