Literature DB >> 15463559

RNA polymerase I-mediated protein-coding gene expression in Trypanosoma brucei.

H M Chung1, M G Lee, L H Van der Ploeg.   

Abstract

Protein-coding genes are transcribed by RNA polymerise (pol) II in all eukaryotes analyzed to date, with the exception of the protozoan Trypanosoma brucei, where pol I can mediate expression of chloramphenicol acetyl transferase (CAT) and neomycin phosphotransferase (neo) reporter genes. The addition of the capped 39-nucleotide (nt) mini-exon to the pre-messenger RNA (mRNA) by trans-splicing in T. brucei has presumably led to the uncoupling of the requirement for production of mRNA by pol II. Here Hui-min Chung, Mary G-S. Lee and Lex Van der Ploeg review the evidence that supports the notion that pol I also transcribes a subset of naturally occurring protein-coding genes in T. brucei.

Entities:  

Year:  1992        PMID： 15463559     DOI： 10.1016/0169-4758(92)90194-7

Source DB:  PubMed          Journal:  Parasitol Today        ISSN： 0169-4758


  24 in total

1.  A single-stranded DNA-binding protein shared by telomeric repeats, the variant surface glycoprotein transcription promoter and the procyclin transcription terminator of Trypanosoma brucei.

Authors:  M Berberof; L Vanhamme; S Alexandre; S Lips; P Tebabi; E Pays
Journal:  Nucleic Acids Res       Date:  2000-01-15       Impact factor: 16.971

2.  Stimuli of differentiation regulate RNA elongation in the transcription units for the major stage-specific antigens of Trypanosoma brucei.

Authors:  L Vanhamme; M Berberof; D Le Ray; E Pays
Journal:  Nucleic Acids Res       Date:  1995-06-11       Impact factor: 16.971

3.  Subnuclear localization of the active variant surface glycoprotein gene expression site in Trypanosoma brucei.

Authors:  I Chaves; J Zomerdijk; A Dirks-Mulder; R W Dirks; A K Raap; P Borst
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-13       Impact factor: 11.205

4.  Physical and transcriptional analysis of the Trypanosoma brucei genome reveals a typical eukaryotic arrangement with close interspersionof RNA polymerase II- and III-transcribed genes.

Authors:  M A Marchetti; C Tschudi; E Silva; E Ullu
Journal:  Nucleic Acids Res       Date:  1998-08-01       Impact factor: 16.971

5.  Activity of a trypanosome metacyclic variant surface glycoprotein gene promoter is dependent upon life cycle stage and chromosomal context.

Authors:  S V Graham; B Wymer; J D Barry
Journal:  Mol Cell Biol       Date:  1998-03       Impact factor: 4.272

6.  Characterization of a transcription terminator of the procyclin PARP A unit of Trypanosoma brucei.

Authors:  M Berberof; A Pays; S Lips; P Tebabi; E Pays
Journal:  Mol Cell Biol       Date:  1996-03       Impact factor: 4.272

7.  Mechanisms of developmental regulation in Trypanosoma brucei: a polypyrimidine tract in the 3'-untranslated region of a surface protein mRNA affects RNA abundance and translation.

Authors:  H R Hotz; C Hartmann; K Huober; M Hug; C Clayton
Journal:  Nucleic Acids Res       Date:  1997-08-01       Impact factor: 16.971

8.  An RNA polymerase II promoter in the hsp70 locus of Trypanosoma brucei.

Authors:  M G Lee
Journal:  Mol Cell Biol       Date:  1996-03       Impact factor: 4.272

9.  Disruption of largest subunit RNA polymerase II genes in Trypanosoma brucei.

Authors:  H M Chung; M G Lee; P Dietrich; J Huang; L H Van der Ploeg
Journal:  Mol Cell Biol       Date:  1993-06       Impact factor: 4.272

10.  A promotor directing alpha-amanitin-sensitive transcription of GARP, the major surface antigen of insect stage Trypanosoma congolense.

Authors:  S V Graham; D Jefferies; J D Barry
Journal:  Nucleic Acids Res       Date:  1996-01-15       Impact factor: 16.971
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