AIM: To explore the dynamical changes of histology, histochemistry, energy metabolism, liver microcirculation, liver function and posttransplant survival of liver graft in rats under different warm ischemia times (WIT) and predict the maximum limitation of liver graft to warm ischemia. METHODS: According to WIT, the rats were randomized into 7 groups, with WIT of 0, 10, 15, 20, 30, 45, 60 min, respectively. The recovery changes of above-mentioned indices were observed or measured after liver transplantation. The graft survival and postoperative complications in each subgroup were analyzed. RESULTS: Liver graft injury was reversible and gradually resumed normal structure and function after reperfusion when WIT was less than 30 min. In terms of graft survival, there was no significant difference between subgroups within 30 min WIT. When WIT was prolonged to 45 min, the recipients' long-term survival was severely insulted, and both function and histological structure of liver graft developed irreversible damage when WIT was prolonged to 60 min. CONCLUSION: The present study indicates that rat liver graft can be safely subjected to warm ischemia within 30 min. The levels of ATP, energy charge, activities of glycogen, enzyme-histochemistry of liver graft and its recovery potency after reperfusion may serve as the important criteria to evaluate the quality of liver graft.
AIM: To explore the dynamical changes of histology, histochemistry, energy metabolism, liver microcirculation, liver function and posttransplant survival of liver graft in rats under different warm ischemia times (WIT) and predict the maximum limitation of liver graft to warm ischemia. METHODS: According to WIT, the rats were randomized into 7 groups, with WIT of 0, 10, 15, 20, 30, 45, 60 min, respectively. The recovery changes of above-mentioned indices were observed or measured after liver transplantation. The graft survival and postoperative complications in each subgroup were analyzed. RESULTS:Liver graft injury was reversible and gradually resumed normal structure and function after reperfusion when WIT was less than 30 min. In terms of graft survival, there was no significant difference between subgroups within 30 min WIT. When WIT was prolonged to 45 min, the recipients' long-term survival was severely insulted, and both function and histological structure of liver graft developed irreversible damage when WIT was prolonged to 60 min. CONCLUSION: The present study indicates that rat liver graft can be safely subjected to warm ischemia within 30 min. The levels of ATP, energy charge, activities of glycogen, enzyme-histochemistry of liver graft and its recovery potency after reperfusion may serve as the important criteria to evaluate the quality of liver graft.
Authors: Charles J Imber; Shawn D St Peter; Inigo Lopez de Cenarruzabeitia; Dave Pigott; Tim James; Richard Taylor; James McGuire; David Hughes; Andrew Butler; Michael Rees; Peter J Friend Journal: Transplantation Date: 2002-03-15 Impact factor: 4.939
Authors: Vincent Donckier; Patricia Loi; Jean Closset; Nathalie Nagy; Eric Quertinmont; Olivier Le Moine; Jacques Devière; Michel Goldman; Michel Gelin; Pierre Gianello Journal: Transplantation Date: 2003-03-27 Impact factor: 4.939
Authors: C Peralta; R Bartrons; A Serafin; C Blázquez; M Guzmán; N Prats; C Xaus; B Cutillas; E Gelpí; J Roselló-Catafau Journal: Hepatology Date: 2001-12 Impact factor: 17.425
Authors: Andrey Belous; Clayton Knox; Ian B Nicoud; Janene Pierce; Christopher Anderson; C Wright Pinson; Ravi S Chari Journal: Transplantation Date: 2003-05-27 Impact factor: 4.939
Authors: Peter Abt; Michael Crawford; Niraj Desai; James Markmann; Kim Olthoff; Abraham Shaked Journal: Transplantation Date: 2003-05-27 Impact factor: 4.939
Authors: Justin D Ellett; Zachary P Evans; Jennifer H Fiorini; Ryan N Fiorini; Julia K Haines; Michael G Schmidt; Kenneth D Chavin Journal: Transplantation Date: 2008-07-27 Impact factor: 4.939