Hande Gulcan1, Cigdem Kuzucu, Riza Durmaz. 1. Department of Pediatrics, Inonu University Faculty of Medicine, Malatya, Turkey. hgulcan@inonu.edu.tr
Abstract
BACKGROUND: Increased nosocomial Stenotrophomonas maltophilia infection rates in newborns, especially in recent years, are a significant cause for concern. These cases are the second case group in the literature to have been identified as nosocomial cross-infection with S. maltophilia in neonates. OBJECTIVE: To investigate the clinical, microbiological, and epidemiologic features of the outbreak caused by S. maltophilia in the neonatal intensive care unit within a period of 7 days. METHODS: Three cases with nosocomial S. maltophilia infection considered to be the result of cross-transmission were prospectively analyzed. Arbitrarily primed polymerase chain reaction (AP-PCR) performed with M13 primer and pulsed-field gel electrophoresis (PFGE) of genomic DNA after digestion with XbaI were used to determine clonal relationship among the isolates. Results S. maltophilia was isolated from the blood cultures of all 3 patients. Molecular typing confirmed that the 3 cases were epidemiologically linked. CONCLUSIONS: Opportunistic pathogens such as S. maltophilia can lead to major problems in neonates. Molecular typing is helpful to improve effective control programs for preventing the spread of the infection.
BACKGROUND: Increased nosocomial Stenotrophomonas maltophilia infection rates in newborns, especially in recent years, are a significant cause for concern. These cases are the second case group in the literature to have been identified as nosocomial cross-infection with S. maltophilia in neonates. OBJECTIVE: To investigate the clinical, microbiological, and epidemiologic features of the outbreak caused by S. maltophilia in the neonatal intensive care unit within a period of 7 days. METHODS: Three cases with nosocomial S. maltophilia infection considered to be the result of cross-transmission were prospectively analyzed. Arbitrarily primed polymerase chain reaction (AP-PCR) performed with M13 primer and pulsed-field gel electrophoresis (PFGE) of genomic DNA after digestion with XbaI were used to determine clonal relationship among the isolates. Results S. maltophilia was isolated from the blood cultures of all 3 patients. Molecular typing confirmed that the 3 cases were epidemiologically linked. CONCLUSIONS: Opportunistic pathogens such as S. maltophilia can lead to major problems in neonates. Molecular typing is helpful to improve effective control programs for preventing the spread of the infection.
Authors: Robert P Ryan; Sebastien Monchy; Massimiliano Cardinale; Safiyh Taghavi; Lisa Crossman; Matthew B Avison; Gabriele Berg; Daniel van der Lelie; J Maxwell Dow Journal: Nat Rev Microbiol Date: 2009-07 Impact factor: 60.633
Authors: Ariadnna Cruz-Córdova; Jetsi Mancilla-Rojano; Víctor M Luna-Pineda; Gerardo Escalona-Venegas; Vicenta Cázares-Domínguez; Christopher Ormsby; Isabel Franco-Hernández; Sergio Zavala-Vega; Mónica Andrés Hernández; Marisol Medina-Pelcastre; Israel Parra-Ortega; Daniela De la Rosa-Zamboni; Sara A Ochoa; Juan Xicohtencatl-Cortes Journal: Front Cell Infect Microbiol Date: 2020-02-18 Impact factor: 5.293