OBJECTIVE: To establish a method for isolating and identifying koumine. METHODS: Koumine was first extracted from Gelsemium alkaloids by using chloroform, which was further separated and purified by column chromatograph, and identified by thin-layer chromatography. RESULTS AND CONCLUSION: Koumine crystals were isolated and purified from Gelsemium alkaloids effectively.
OBJECTIVE: To establish a method for isolating and identifying koumine. METHODS:Koumine was first extracted from Gelsemium alkaloids by using chloroform, which was further separated and purified by column chromatograph, and identified by thin-layer chromatography. RESULTS AND CONCLUSION:Koumine crystals were isolated and purified from Gelsemium alkaloids effectively.