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Literature DB >> 154412

A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells.

Abstract

Lymphocytes of rabbits can be separated from small quantities of heparinized whole blood using a simple density gradient of Ficoll-Ronpacon 1.09. This separation technique yields a pure suspension of viable cells allowing reproducible results from cultures stimulated either with PHA or allogeneic lymphocytes isolated by the same technique.

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Year: 1979 PMID： 154412 DOI： 10.1007/bf01920665

Source DB: PubMed Journal: Experientia ISSN： 0014-4754

11 in total

1. One-way nonstimulation of mixed leukocyte culture in dog families.

Authors: E Gluckman; M L Schroeder; R Storb; H Goselink; S Johnson; T C Graham; G Pretorius; E D Thomas
Journal: Transplantation Date: 1975-01 Impact factor: 4.939

2. Further miniaturization and automation of in vitro lymphocyte cultures.

Authors: V C Miggiano; T Meo; I Bergén; M Nabholz
Journal: Tissue Antigens Date: 1975-04

3. Marrow grafts between canine litter-mates homozygous or heterozygous for lymphocyte-defined histocompatibility antigens.

Authors: R Storb; P L Weiden; M L Schroeder; T C Graham; K G Lerner; E D Thomas
Journal: Transplantation Date: 1976-04 Impact factor: 4.939

4. Cytofluorometric analysis of the kinetics of lymphocyte transformation after phytohemagglutinin stimulation: comparison with the kinetics of thymidine incorporation.

Authors: P D Utsinger; W J Yount; J G Fallon; M J Logue; C R Fuller; D Elliott
Journal: Blood Date: 1977-01 Impact factor: 22.113

A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells.

1. One-way nonstimulation of mixed leukocyte culture in dog families.

2. Further miniaturization and automation of in vitro lymphocyte cultures.

3. Marrow grafts between canine litter-mates homozygous or heterozygous for lymphocyte-defined histocompatibility antigens.

4. Cytofluorometric analysis of the kinetics of lymphocyte transformation after phytohemagglutinin stimulation: comparison with the kinetics of thymidine incorporation.

5. Histocompatibility in the rabbit. Identification of the major locus.

6. Histocompatibility in the rabbit. Linkage between RL-A, MLC, and the He blood group loci.

7. Cell-mediated lympholysis in HL-A identical unrelated individuals.

8. Studies on histocompatibility in skin allotransplantaion in rabbits.

9. Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report.

10. Rabbits possess conventional T lymphocytes.

1. Cyclosporine resistant effector cells in rabbit skin allografts.