Literature DB >> 1543730

Uptake and metabolism of a fluorescent sulfatide analogue in cultured skin fibroblasts.

E Monti1, A Preti, A Novati, M F Aleo, M L Clemente, S Marchesini.   

Abstract

The sulfatide fluorescent analogue N-lissamine rhodaminyl-(12-aminododecanoyl) cerebroside 3-sulfate was administered in the form of albumin complex to normal human skin fibroblasts and its metabolic fate was investigated. Ceramide, galactosylceramide, glucosylceramide, sphingomyelin and free acid, all containing the fluorophore lissamine rhodamine, have been synthesized as reference standards for the identification of the metabolic products. Ceramide appeared to be the main metabolic product present both in cell extract and medium, followed by galactosylceramide and sphingomyelin. Fluorescence microscopy of cells showed a marked perinuclear fluorescence.

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Year:  1992        PMID： 1543730     DOI： 10.1016/0005-2760(92)90129-j

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Use of lissamine rhodamine ceramide trihexoside as a functional assay for alpha-galactosidase A in intact cells.

Authors:  Christine R Kaneski; Raphael Schiffmann; Roscoe O Brady; Gary J Murray
Journal:  J Lipid Res       Date:  2010-06-06       Impact factor: 5.922

2.  High residual arylsulfatase A (ARSA) activity in a patient with late-infantile metachromatic leukodystrophy.

Authors:  J Kreysing; W Bohne; C Bösenberg; S Marchesini; J C Turpin; N Baumann; K von Figura; V Gieselmann
Journal:  Am J Hum Genet       Date:  1993-08       Impact factor: 11.025

3.  Globotriaosylceramide induces oxidative stress and up-regulates cell adhesion molecule expression in Fabry disease endothelial cells.

Authors:  Jin-Song Shen; Xing-Li Meng; David F Moore; Jane M Quirk; James A Shayman; Raphael Schiffmann; Christine R Kaneski
Journal:  Mol Genet Metab       Date:  2008-08-15       Impact factor: 4.797

4.  Functional compartments of sulphatide metabolism in cultured living cells: evidence for the involvement of a novel sulphatide-degrading pathway.

Authors:  M C Tempesta; R Salvayre; T Levade
Journal:  Biochem J       Date:  1994-02-01       Impact factor: 3.857

5.  Novel patient cell-based HTS assay for identification of small molecules for a lysosomal storage disease.

Authors:  Haifeng Geng; Grace Whiteley; Jameson Ribbens; Wei Zheng; Noel Southall; Xin Hu; Juan J Marugan; Marc Ferrer; Gustavo H B Maegawa
Journal:  PLoS One       Date:  2011-12-21       Impact factor: 3.240

6.  Laminin-sulfatide binding initiates basement membrane assembly and enables receptor signaling in Schwann cells and fibroblasts.

Authors:  Shaohua Li; Patricia Liquari; Karen K McKee; David Harrison; Raj Patel; Sean Lee; Peter D Yurchenco
Journal:  J Cell Biol       Date:  2005-04-11       Impact factor: 10.539

7.  Generation of an in vitro model for peripheral neuropathy in Fabry disease using CRISPR-Cas9 in the nociceptive dorsal root ganglion cell line 50B11.

Authors:  Christine R Kaneski; John A Hanover; Ulrike H Schueler Hoffman
Journal:  Mol Genet Metab Rep       Date:  2022-04-27
  7 in total

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