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Literature DB >> 1542573

The synthesis and properties of oligodeoxyribonucleotides containing N6-methoxyadenine.

Abstract

Oligodeoxyribonucleotides containing N6-methoxyadenine (M) have been synthesized. The order of stability of duplexes consisting of synthesized oligodeoxyribonucleotides, 5'd(CCTGGTAXCAGGTCC)3'-5'd(GGACCTGNTACCAGG)3' (X = M, A, G. N = A, G, T, C), was M: A (Tm = 52 degrees C) greater than M: T (50 degrees C) greater than M: G (48 degrees C) greater than M: C (46 degrees C) observed by thermal denaturation in a buffer of 0.01 M Na cacodylate, and 0.1 M NaCl at pH 7.0. The Tms are within a range of 6 degrees of difference, which is smaller than those of Tms of the duplexes containing A:N pairs (11 degrees) and G:N pairs (11 degrees). DNA replication study on a template-primer system, 5'd(32p-CAGCTTTCGC)3' 3'd(GTCGAAAGCGMAGTCG)5', showed that TTP and dCTP were incorporated into DNA strands at a site opposite to M by Klenow DNA polymerase, but dATP and dGTP were not.

Entities: Chemical

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Year: 1992 PMID： 1542573 PMCID： PMC312017 DOI： 10.1093/nar/20.4.777

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

19 in total

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Journal: Nucleic Acids Res Date: 1986-10-24 Impact factor: 16.971

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Journal: Nucleic Acids Res Date: 1985-07-11 Impact factor: 16.971

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Authors: B Singer; J T Kuśmierek
Journal: Annu Rev Biochem Date: 1982 Impact factor: 23.643

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Authors: G I Birnbaum; B Kierdaszuk; D Shugar
Journal: Nucleic Acids Res Date: 1984-03-12 Impact factor: 16.971

7. NMR studies on oligodeoxyribonucleotides containing the dam methylation site GATC. Comparison between d(GGATCC) and d(GGm6ATCC).

Authors: G V Fazakerley; R Téoule; A Guy; H Fritzsche; W Guschlbauer
Journal: Biochemistry Date: 1985-08-13 Impact factor: 3.162

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Authors: N N Anand; D M Brown; S A Salisbury
Journal: Nucleic Acids Res Date: 1987-10-26 Impact factor: 16.971

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Journal: Acta Biochim Pol Date: 1984 Impact factor: 2.149

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Journal: J Biol Chem Date: 1986-02-15 Impact factor: 5.157

4 in total

1. Comparative mutagenicities of N6-methoxy-2,6-diaminopurine and N6-methoxyaminopurine 2'-deoxyribonucleosides and their 5'-triphosphates.

Authors: F Hill; D M Williams; D Loakes; D M Brown
Journal: Nucleic Acids Res Date: 1998-03-01 Impact factor: 16.971