Literature DB >> 154056

Sterol content and enzyme defects of nystatin-resistant mutants of Neurospora crassa.

M Grindle, R Farrow.   

Abstract

Non-saponifiable cell extracts of wild type and sterol mutants of N. crassa were analysed by means of gas-liquid chromatography. The wild-type contained ergosterol and episertol in a 10:1 ratio. None of the mutants was able to synthesize ergosterol. Three of the mutants carry single recessive gene mutations cauisng blocks in the terminal steps of ergosterol biosynthesis: erg-1 has an inactive delta 8 leads to delta 7 isomerase erg-2 has an inactive 24(28) hydrogenase, and erg-4 has an inactive C-24 methyl transferase. Some of the mutants accumulated novel sterols as a result of their enzyme defects. The genes erg-1 and erg-2 were mapped close to inl on the right arm of chromosome V.

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Year:  1978        PMID： 154056     DOI： 10.1007/bf00332531

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  8 in total

1.  Biosynthesis of terpenes and steroids. X. The sterols of some yeast mutants doubly defective in ergosterol biosynthesis.

Authors:  D H Barton; A A Gunatilaka; T R Jarman; D A Widdowson; M Bard; R A Woods
Journal:  J Chem Soc Perkin 1       Date:  1975

2.  Biosynthesis of terpenes and steroids. IX. The sterols of some mutant yeasts and their relationship to the biosynthesis of ergosterol.

Authors:  D H Barton; J E Corrie; M Bard; R A Woods
Journal:  J Chem Soc Perkin 1       Date:  1974

3.  Investigations on the biosynthesis of steroids and terpenoids. VI. The sterols of yeast.

Authors:  D H Barton; U M Kempe; D A Widdowson
Journal:  J Chem Soc Perkin 1       Date:  1972

4.  Studies on the accumulation of ergosterol and 24(28)-dehydroergosterol in 3 strains of Saccharomyces cerevisiae.

Authors:  R A Woods; M Bard; I E Gardner; S W Molzahn
Journal:  Microbios       Date:  1974 Jun-Jul

5.  The efficacy of various mutagens and polyene antibiotics for the induction and isolation of sterol mutants of Neurospora crassa.

Authors:  M Grindle
Journal:  Mol Gen Genet       Date:  1974-04-09

6.  Sterol mutants of Neurospora crassa: their isolation, growth characteristics and resistance to polyene antibiotics.

Authors:  M Grindle
Journal:  Mol Gen Genet       Date:  1973-02-02

7.  Detection of the ergosterol and episterol isomers lichesterol and fecosterol in nystatin-resistant mutants of Neurospora crassa.

Authors:  D C Morris; S Safe; R E Subden
Journal:  Biochem Genet       Date:  1974-12       Impact factor: 1.890

8.  Nystatin-resistant mutants of yeast: alterations in sterol content.

Authors:  R A Woods
Journal:  J Bacteriol       Date:  1971-10       Impact factor: 3.490
  8 in total
  3 in total

Review 1.  Chromosomal loci of Neurospora crassa.

Authors:  D D Perkins; A Radford; D Newmeyer; M Björkman
Journal:  Microbiol Rev       Date:  1982-12

2.  Plasma Membrane Fusion Is Specifically Impacted by the Molecular Structure of Membrane Sterols During Vegetative Development of Neurospora crassa.

Authors:  Martin Weichert; Stephanie Herzog; Sarah-Anne Robson; Raphael Brandt; Bert-Ewald Priegnitz; Ulrike Brandt; Stefan Schulz; André Fleißner
Journal:  Genetics       Date:  2020-10-12       Impact factor: 4.562

3.  Abnormal Ergosterol Biosynthesis Activates Transcriptional Responses to Antifungal Azoles.

Authors:  Chengcheng Hu; Mi Zhou; Wenzhao Wang; Xianyun Sun; Oded Yarden; Shaojie Li
Journal:  Front Microbiol       Date:  2018-01-17       Impact factor: 5.640
  3 in total

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