BACKGROUND: The quantity of circulating cell-free Epstein-Barr virus (EBV) DNA in patients with nasopharyngeal carcinoma (NPC) managed by radiotherapy has prognostic relevance. We measured the copy number of EBV DNA in patients with early recurrent NPC before and after salvage nasopharyngectomy. METHODS: Nasopharyngectomy with the maxillary swing approach was performed for 28 patients. Serum blood samples were taken prospectively before nasopharyngectomy and on postoperative day 7. Plasma cell-free EBV DNA copies were measured with a real-time quantitative polymerase chain reaction for the BamHI-W fragment of the EBV genome. RESULTS: Cell-free EBV DNA was detected in 17 patients before nasopharyngectomy. Surgical resection reduced the copy number of EBV DNA significantly (p = .016). Negative surgical margins achieved during nasopharyngectomy is associated with a zero EBV DNA copy postoperatively (p = .022). CONCLUSION: Cell-free EBV DNA was detected in 61% of patients with recurrent NPC, and its quantity postoperatively reflects whether the salvage nasopharyngectomy has achieved a negative surgical margin.
BACKGROUND: The quantity of circulating cell-free Epstein-Barr virus (EBV) DNA in patients with nasopharyngeal carcinoma (NPC) managed by radiotherapy has prognostic relevance. We measured the copy number of EBV DNA in patients with early recurrent NPC before and after salvage nasopharyngectomy. METHODS: Nasopharyngectomy with the maxillary swing approach was performed for 28 patients. Serum blood samples were taken prospectively before nasopharyngectomy and on postoperative day 7. Plasma cell-free EBV DNA copies were measured with a real-time quantitative polymerase chain reaction for the BamHI-W fragment of the EBV genome. RESULTS: Cell-free EBV DNA was detected in 17 patients before nasopharyngectomy. Surgical resection reduced the copy number of EBV DNA significantly (p = .016). Negative surgical margins achieved during nasopharyngectomy is associated with a zero EBV DNA copy postoperatively (p = .022). CONCLUSION: Cell-free EBV DNA was detected in 61% of patients with recurrent NPC, and its quantity postoperatively reflects whether the salvage nasopharyngectomy has achieved a negative surgical margin.
Authors: Aparna A Kamat; Mathew Baldwin; Diana Urbauer; Diana Dang; Liz Y Han; Andrew Godwin; Beth Y Karlan; Joe L Simpson; David M Gershenson; Robert L Coleman; Farideh Z Bischoff; Anil K Sood Journal: Cancer Date: 2010-04-15 Impact factor: 6.860
Authors: J S Kalpoe; P B Douwes Dekker; J H J M van Krieken; R J Baatenburg de Jong; A C M Kroes Journal: J Clin Pathol Date: 2006-02-17 Impact factor: 3.411
Authors: Katarina Kalavska; Tomas Minarik; Barbora Vlkova; Denisa Manasova; Michaela Kubickova; Andrej Jurik; Jozef Mardiak; Jozef Sufliarsky; Peter Celec; Michal Mego Journal: J Ovarian Res Date: 2018-09-22 Impact factor: 4.234