| Literature DB >> 15381182 |
Tom Sprong1, Dominic L Jack, Nigel J Klein, Malcolm W Turner, Peter van der Ley, Liana Steeghs, Liesbeth Jacobs, Jos W M van der Meer, Marcel van Deuren.
Abstract
Mannose binding lectin (MBL) is a key molecule in the lectin pathway of complement activation, and likely of importance in our innate defence against meningococcal infection. We evaluated the role of MBL in cytokine induction by LPS or non-LPS components of Neisseria meningitidis, using a meningococcal mutant deficient for LPS. Binding experiments showed that MBL exhibited low, but significant binding to encapsulated LPS+ meningococci (H44/76) and LPS-deficient (LPS-) meningococci (H44/76lpxA). Experiments with human mononuclear cells (PBMCs) showed that MBL significantly augmented IL-1beta production after stimulation with LPS+ and LPS- meningococci, in a dose-dependent fashion. In addition, IL-10 production was enhanced after stimulation with LPS- meningococci. In contrast, TNFalpha, IL-6 and IFNgamma productions were unaffected. No effect of MBL was observed on cytokine induction by meningococcal LPS. MBL enhanced cytokine production at concentrations >10(7) meningococci. It is concluded that MBL interacts with non-LPS components of N. meningitidis and in this way modulates the cytokine response.Entities:
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Year: 2004 PMID： 15381182 DOI： 10.1016/j.cyto.2004.06.007
Source DB: PubMed Journal: Cytokine ISSN： 1043-4666 Impact factor: 3.861